【摘要】 利用电喷雾离子阱质谱（ESI-IT-MS）技术,对8个具有潜在杀菌、抗癌生物活性的三类新型N-苯甲酰基-脱氢枞胺衍生物进行了ESI质谱行为研究。结果表明,由于7位、12位及苯甲酰基上取代基的不同,三类衍生物呈现出一定的电离及碎裂特性。在（±）-ESI-MS过程中,7-肟基类衍生物（A）和12-硝基类衍生物（C）比7-羟基类衍生物（B）更易产生二聚体;A易产生[M-H₂O+H]⁺,C则更易负离子化。在（+）-ESIMS/MS过程中,A中7-肟基的存在使其易产生特征离子碎裂峰[M-35+H]⁺和[M-H₂O+H]⁺;A的18位酰基脱水离子峰[M-H₂O+H]⁺失去一分子取代苯甲腈产生系列偶数碎裂离子峰;A的7-肟基脱水离子峰[M-H₂O+H]⁺失去一分子取代苯甲醛产生系列奇数碎裂离子峰。在（-）-ESI-MS/MS过程中,B比C更稳定,在较高的碰撞能作用下获得的碎裂片段较少;均可通过丢失中性分子N-取代苯甲酰基亚甲胺、取代苯甲醛或取代苯产生系列碎裂离子片段;C的强吸电子基团12-硝基及4’-硝基的存在使其丢失的中性分子易捕获电子而离子化。更多还原

【Abstract】 Eight novel N-benzoyl dehydroabietylamine derivatives with potential sterilization and anticancer biological activities were analyzed through electrospray ionization tandem mass spectrometry（ESI-ITMS）.The results indicated that three types of derivatives exhibited their own typical ionization and fragmentation process,due to their different 7-and 12-substituents on the dehydroabietylamine ring and different substituents on the benzoyl ring.In（±）-ESI-MS,the dimer ions of 7-oxime derivatives（A） and12-nitro derivatives（C） could be more readily formed than that of 7-hydroxy derivatives（B）.[M-H₂O+ H]⁺ of A was observed in the positive-ion mode.C were prone to be ionized in the negative-ion mode.Two typical fragmentation peaks of[M-35+H]⁺ and[M-H₂O + H]⁺ were observed in（+）-ESIMS/MS spectra of 7-oxime dehydroabietylamine derivatives.The ion of[M- H₂O + H]⁺ formed from18-acyl of（A） could induce some even fragmentation ion peaks by loss of substituted benzonitrile.The ion of[M-H₂O + H]⁺ formed from 7-oxime of（A） can induce some odd fragmentation ion peaks by loss of substituted benzaldehyde.（B） showed more stability than（C） in（-）-ESI-MS/MS process.Fewer fragmentation ions of（B） were obtained even though（B） derivatives were collided with higher energy.Ion fragmentation of（B） and（C） derivatives were induced by loss of neutral molecules of N-substituted benzoyl methylene amine,substituted benzaldehyde or substituted benzene.Due to the strong electron-withdrawing ability of 12-and 4’-nitro of（C） derivatives,the neutral molecules lost from（C） derivatives can be ionized by capturing one electron.更多还原