【摘要】 目的促进宁夏不同地区人兽共患病自然疫源地宿主动物的准确分类和鉴定,提高形态分类的准确性,探讨DNA条形码技术分型的可行性。方法在宁夏不同地区及生境采集啮齿动物标本154份,扩增标本的COI基因部分序列,并进行测序和相关分析。结果捕获的19个形态种的啮齿类动物中,通过COI基因的分析发现20个分子种。形态上鉴定为黑线仓鼠的标本存在2个分子种;贺兰山鼠兔在分子上与褐斑鼠兔比较接近,遗传距离为3.6%;结论 DNA条形码可以用于啮齿动物标本的准确鉴定,纠正形态鉴定的错误;可以发现形态无法区分的隐含种,可以更好的研究啮齿类的分类及进化关系。更多还原

【Abstract】 To improve the ability of correct classification and identification of rodents in different kinds of foci of zoonosis in Ningxia Hui Autonomous Region in China,rodents samples from different habitats of Ningxia were collected.Sequences of COI gene were amplified and sequenced from 154 samples.Based on these sequences,the pairwise genetic distance were calculated,and a Neighbor Joining tree were constructed.According to the NJ tree,20 clusters with high bootstrap support were found from 19 morphological species.The striped dwarf hamsters were divided into two clusters,which suggested that there were two cryptic species with stripe on the back.The pika from Helan Mountain showed close relationship with Ochotona pallasi,and the genetic distance was as low as 3.6%.Results show that DNA barcodes could be used to accurately identify specimens of rodents and correct morphological identification errors.It could discover appearance indistinguishable implied species and could better study the classification and evolution of rodents.更多还原