Objective To develop an HPLC method for the content determination of notopterol, isoimperatorin, costunolide and dehydrocostuslactone in Chenxiangqu. Methods An Hypersil C18 column was used as the chromatographic column, and the flow rate was 0.9 mL · min- 1. Notopterol and isoimperatorin: the mobile phase consisted of acetonitrile-0.5% phosphoric acid solution(67 :33). The UV detection wavelength was 312 nm. Costunolide and dehydrocostuslactone: the mobile phase consisted of acetonitrile-methanol-1% acetic...