【摘要】 为阐明贪铜菌6-5的耐镉机理,更有效的治理土壤重金属污染。本研究利用同源序列克隆技术从本研究室筛选的高耐镉菌株贪铜菌6-5中成功克隆出双组分信号转导系统czc R2-czc S2,通过DNA测序及序列比对,结果表明,该扩增产物的DNA序列与耐金属贪铜菌CH34中megaplasmid质粒的czc R2和czc S2基因的同源性分别为99%和98%。czc R2-czc S2彼此相邻,并且转录方向一致,很可能作为一个独立的操纵子控制其他功能基因的表达。并利用生物信息学对该调控基因的一般特性、跨膜区和信号肽进行了预测,该研究为开展更为深入的研究以及制定有针对性的重金属污染治理对策建立了基础,具有重要的理论意义和实际应用潜能。更多还原

【Abstract】 In order to elucidate the Cd-resistance mechanism of Cupriavidus 6-5 and reduce the heavy metalpollution more efficiently. The two- component signal transduction system czc R2- czc S2 from high Cd-resistance strain Cupriavidus 6-5 was successfully cloned by homology-based cloning method. Results of DNAsequencing and sequence alignment showed that the identity of the nucleotide sequence of obtained genesczc R2 and czc S2 between Cupriavidus 6- 5 and the megaplasmid of Cupriavidus metallidurans CH34 werefrom 99% and 98%, respectively. czc R2 and czc S2, adjacent to each other and the same direction oftranscription, control other functional gene expression probably as an independent operon. The generalcharacteristics, signal peptide and transmembrane domain of the regulatory gene were predicted by thebioinformatics. The study establish foundation for the development of more thorough research and making ofheavy metal pollution control countermeasure, the work has the theory significance and actual applicationpotential.更多还原