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广西珍稀野生茶树叶片解剖结构分析

Study on Anatomy Structures of Leaves of Rare Wild Camellia sinensis from Guangxi

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【作者】 李凤英陈佳邓慧群覃秀菊陈凤罗小梅

【Author】 Li Fengying;Chen Jia;Deng Huiqun;Qin Xiuju;Chen Feng;Luo Xiaomei;College of Life Science, Guangxi Normal University;Tea Science and Research Institute of Guangxi;Guangxi Institute of Excellent Tea Varieties;Dadong Middle School of Bobai County;

【机构】 广西师范大学生命科学学院广西桂林茶叶科学研究所广西优异茶树品种研究院广西博白县大垌镇初级中学

【摘要】 采用石蜡切片方法和光学显微技术,对广西珍稀野生茶10个株系叶片进行解剖和比较观察。从解剖学角度分析比较其抗旱、抗寒和抗病虫害能力,为广西亚热带特异茶树资源的鉴定与新品种的选育以及种质资源利用提供理论依据。试验结果表明:叶脉、叶片、表皮细胞、角质层、栅栏组织及海绵组织的厚度因株系的不同而有所不同。上表皮细胞厚度为17.01~29.34μm,上表皮角质厚度为2.10~5.76μm,栅栏组织/海绵组织厚度比值为0.22~0.85,叶脉/叶片厚度比值为2.46~4.66。分析结果显示:其中抗旱、抗寒和抗病虫等综合抗性最强的是JB2号和JB5号,次强的是JB1号、JB3号和JL1号;这5个株系均为抗逆性较强的茶树资源,可入选为高抗品种的育种材料并进一步鉴定。

【Abstract】 The anatomy structures of leaves of 10 strains of wild Camellia sinensis from Guangxi were studiedvia paraffin-sectioning and optical microtechnique. The study based on the anatomy could help us to explorethe ability of anti-drought, anti-cold, and anti-pest, which provided the scientific foundation to the tropical tearesource identifying and new varieties cultivating. The results showed that the thickness of several structures,including blade, vein, epidermis cells, cuticula, fence form and sponge form significantly varied according tostrain. The thickness of the upper epidermic cells ranged from 17.01 μm to 29.34 μm, and the upperepidermic cuticula ranged from 2.10 μm to 5.76 μm. The ratio of thickness of fence form to sponge form rangedfrom 0.22 to 0.85, while the ratio of vein to blade ranged from 2.46 to 4.66. Further analysis indicated that JB2and JB5 exhibited strongest ability in anti-cold, drought and pest, followed by JB1, JB3, JL1, respectively,which were considered as the top 5 strain of wild Camellia sinensis with stronger resistance.

【关键词】 野生茶树解剖结构叶片
【Key words】 wild Camellia sinensisanatomy structuresleaves
【基金】 广西科技开发国际合作项目“广西亚热带特异茶树资源的快速鉴定与选育新技术研究”(桂科合10100019-13);桂林市科技开发项目“广西亚热带特异茶树资源的快速鉴定与选育新技术研究”(20100314);广西师范大学项目“石蜡切片实验方法的改进及其在探究实验中的应用”(2012GJY22)
  • 【文献出处】 中国农学通报 ,Chinese Agricultural Science Bulletin , 编辑部邮箱 ,2014年13期
  • 【分类号】S571.1
  • 【被引频次】8
  • 【下载频次】286
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