【摘要】 目的:联合检测抗突变型瓜氨酸波形蛋白(anti-MCV)抗体、抗环瓜氨酸多肽(anti-CCP)抗体和类风湿因子(RF-IgM)水平,探讨anti-MCV在类风湿关节炎(RA)中的诊断价值。方法:采用酶联免疫吸附试验法检测359例RA患者(RA患者组)、128例其他风湿性疾病患者(疾病对照组)和90例健康体检者(正常对照组)的血清anti-MCV和anti-CCP水平,并采用动态免疫散射比浊法测定血清中RF-IgM含量,从实验数据中得到各实验指标的灵敏度和特异性。结果:AntiMCV、anti-CCP和RF-IgM对RA的诊断灵敏度分别为85.1%、76.7%和82.7%,特异性分别为93.2%、95.1%和80.1%。联合检测anti-MCV和anti-CCP,灵敏度降低到70.2%;但特异性增加到98.7%。如同时检测anti-MCV和anti-CCP,并以二者中任何一种指标为阳性标准,那么,对RA的灵敏度和特异性分别为89.5%和97.6%。结论:anti-MCV和anti-CCP是诊断RA的高灵敏度和高特异性的检测指标,联合检测anti-MCV和anti-CCP较单一检测anti-MCV或anti-CCP具有更好的RA诊断价值。更多还原

【Abstract】 Objective: Combined detection of anti-mutated citrullinated vimentin( anti-MCV) antibodies,anti-cyclic citrullinated peptide( anti-CCP) antibodies and rheumatoid factor( RF-IgM) levels to investigate the diagnostic value of anti-mutated citrullinated vimentin( anti-MCV) antibodies for rheumatoid arthritis( RA). Methods: A total of 359 patients with RA,128 patients with other rheumatic diseases and 90 healthy controls were involved. Enzyme linked immunosorbent assay( ELISA) was used to detect anti-MCV and anti-CCP,and dynamic immune nephelometry was applied to detect RF-IgM. The sensitivity and specificity were obtained from the experimental data. Results: The sensitivities of anti-MCV,anti-CCP and RF-IgM were 85. 1%,76. 7% and 82. 7% in RA respectively. The specificities were 93. 2%,95. 1% and 80. 1% respectively. Combined detection of anti-MCV and anti-CCP,the sensitivity decreased to 70. 2%; but the specificity increased to 98. 7%. The sensitivity reached to 89. 5% with specificity 97. 6% when the union of anti-MCV and anti-CCP positivity was used as criterion. Conclusion: Anti-MCV and anti-CCP are novel makers for RA diagnosis with high sensitivity and high specificity. Combination of anti-MCV and anti-CCP is more helpful for RA diagnosis.更多还原