【摘要】 目的:观察染料木素(genistein)对人非小细胞型肺癌(non small cell lung cancer,NSCLC)A549/DDP细胞增殖和凋亡的影响。方法:培养A549及A549/DDP细胞株,以A549细胞为对照。①MTT法测定A549及A549/DDP细胞对顺铂的IC50值、耐药倍数及细胞增殖抑制率;②测定0、1.25、2.5、5.0、10、20、40、60、80μg/ml染料木素作用48 h对A549/DDP细胞增殖的抑制率及IC50值;③用6.25、12.5、25μg/ml染料木素处理A549/DDP细胞24 h后,经流式细胞计量仪检测细胞周期及细胞凋亡情况。结果:①A549及A549/DDP细胞对顺铂的IC50值分别为33.6μmol/L和76.9μmol/L,耐药倍数为2.3;细胞增殖抑制率随顺铂浓度增加而逐渐加大;②染料木素对A549/DDP细胞生长的影响,随染料木素浓度增加表现为先促增殖后抑制的作用,其对A549及A549/DDP细胞的IC50值分别为85.1μg/ml和80.2μg/ml;③6.25、12.5、25μg/ml染料木素作用于A549/DDP细胞24 h后,随染料木素浓度的增加,停留于G2/M期的细胞数逐渐增多(P<0.05),同时A549/DDP细胞出现凋亡。结论:染料木素可抑制A549/DDP细胞的生长,将细胞阻滞于G2/M期,并诱导细胞凋亡。更多还原

【Abstract】 Objective:To observe the effects of genistein on proliferation and apoptosis of human non-small cell lung cancer cell line A549 / DDP. Methods: ①MTT assay was applied to evaluate the resistance index of A549 / DDP cell line to cisplatin and half inhibitory concentration( IC50). ②Inhibition rate of A549/DDP cell proliferation and IC50value were evaluated by MTT assay after treatment with 0,1. 25,2. 5,5. 0,10,20,40,60,80 μg / ml genistein for 48 hour respectively. ③A549 / DDP cell cycle and apoptosis were evaluated by flow cytometry after treatment with 6. 25,12. 5,25 μg / ml genistein for 24 hours respectively. Results: ①In exposing to cisplatin,the IC50of A549 and A549 / DDP was 33. 6 μmol / L and 76. 9 μmol / L respectively. The resistance index was 2. 3. Cell growth inhibition rate increased following the cisplatin concentration increasing gradually. ②A549 / DDP growth inhibition rate increased at first and later decreased gradually following treatment with the genistein dose increased. The IC50of A549 and A549 / DDP was about 85. 1 μg / ml and 80. 2 μg / ml respectively. ③After treatment with 6. 25,12. 5,25 μg / ml genistein for 24 hours,there were more A549 / DDP cells arresting and showing apoptosis along with the genistein dose increased. Conclusion: Genistein can inhibit A549 / DDP proliferation,cause A549 / DDP arresting in G2 / M phase and induce A549 / DDP cell apoptosis with dose dependently.更多还原