利用毕赤酵母GS115表达白地霉的脂肪酶基因,以离子交换法纯化的重组脂肪酶催化菜籽油水解,确定了最佳水解条件为温度42℃,水油比为1.5∶1,p H 8.0,酶用量为250 U/g,反应18 h水解率可达到40.1%。以水解得到的混合脂肪酸为原料,建立一种尿素包合法富集α-亚麻酸的工艺。通过单因素试验对影响尿素包合富集效果的包合温度、时间、溶剂配比进行研究,由正交试验得到包合过程的最佳工艺条件为m(脂肪酸)∶m(尿素)∶V(乙醇)为1∶4∶20,包合温度-20℃、包合时间14 h,此条件可使其中α-亚麻酸的含量由8.03%提高至19.24%。
【英文摘要】
The gene coding the lipase of Geotrichum candidum was expressed in Pichia pastoris GS115. The hydrolysis of rapeseed oil was catalyzed with recombinant lipase purified by one single step of ion-exchange. The optimum enzymatic hydrolysis conditions were determined as follows: temperature is 42 ℃,ratio of water to oil is 1. 5∶ 1,with p H 8. 0 and lipase dosage 250 U / g,the reaction time is 18 h and the hydrolysis ratio is 40. 1%. The mix-fatty acids obtained by the hydrolysis were used as raw material and a ...
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