【摘要】 目的研究胎鼠间充质干细胞(MSCs)移植对老年小鼠肺功能的影响。方法将12月龄雌性BALB/c雌性老年小鼠随机分为对照组、移植组。将从孕13.5 d胎鼠分离培养MSCs,经尾静脉注射到移植组小鼠。观察移植后3个月小鼠的一般情况,经超声心动图检查评价肺动脉血流速度、左心功能的变化,进行负重游泳试验,测量游泳前后全血乳酸变化及肺组织的病理学变化。结果与对照组相比,移植组小鼠肺组织病理学观察发现老年肺气肿样改变明显减轻;肺动脉血流速度较慢,每搏输出量、心输出量、左室舒张期容积均增加;负重游泳时间明显延长,游泳前后全血乳酸水平降低。结论移植胎鼠MSCs能够延缓老年小鼠肺脏的退行性变化,改善老年小鼠肺功能。更多还原

【Abstract】 Objective To investigate the effect of mouse fetus-derived mesenchymal stem cells( MSCs) transplantation on lung function in aged mice. Methods The 12-month-old BALB /c mice were randomly divided into control and transplantation groups. MSCs were derived from 13. 5 d fetuses. The mice in transplantation group were injected with fetus-derived MSCs through the tail vein,while mice in control group were injected with equal volume of normal saline. Morphologic analysis of the lung tissue was performed. The pulmonary artery blood flow velocity and cardiac function were measured by echocardiography. Results There were the senile emphysema changes in control group,MSCs significantly ameliorated the senile emphysema changes in transplanted mice. Pulmonary artery blood flow velocity in transplantation group was slow down,and the stroke volume,cardiac output and left ventricular diastolic volume were increased. Weight-loaded swimming time of MSCs transplantation group was longer,blood lactic acid levels before and after swimming were lower than those in control group. Conclusions Transplantation of mouse fetus-derived MSCs could delay degenerative changes of the lung,improve the lung function of the aged mice.更多还原