【摘要】 目的观察不同缺血时间所引起的再灌注损伤小鼠脑神经细胞Bcl-2、Caspase-3蛋白表达的变化。方法健康昆明种小鼠50只,随机均分为五组,假手术组:仅行手术,不实施脑缺血;I/RⅠ组:缺血30 min,再灌注1 h;I/RⅡ组:缺血60 min,再灌注1 h;I/RⅢ组:缺血90 min,再灌注1 h;I/RⅣ组:缺血120 min,再灌注1 h;取小鼠脑组织,进行免疫组织化学染色(SP法)和计算机图像分析。结果在一定缺血时间范围内,Bcl-2随缺血时间的延长而表达增加,其中I/RⅡ组Bcl-2的平均光密度(MOD)值分别与假手术组和I/RⅠ组比较,均有显著性差异(P<0.05);但缺血时间进一步延长,I/RⅢ组和I/RⅣ组Bcl-2表达逐渐减少,分别与I/RⅡ组Bcl-2的MOD值比较,均有显著性差异(P<0.05)。Caspase-3随着缺血时间的延长而表达不断增加,I/RⅠ组、I/RⅡ组和I/RⅢ组Caspase-3的MOD值分别与假手术组比较,其差异均有显著性(P<0.05);但缺血时间过久,I/RⅣ组Caspase-3表达减弱,与I/RⅢ组Caspase-3的MOD值比较,有显著性差异(P<0.05)。结论在缺血早期受损神经元内,Bcl-2和Caspase-3表达均上调且Caspase-3占优势,但随着缺血时间的进一步延长,Bcl-2表达显著减少,为临床上早期使用Caspase抑制剂和Bcl-2激动剂治疗急性脑缺血性疾病提供实验室依据。更多还原

【Abstract】 Objective To observe the expression and significance of Bcl-2 and Caspase-3 in brain neurons cells of the different ischemia reperfusion injury of mice and for the treatment and prognosis of acute cerebral ischemic diseases clinically provide laboratory basis. Methods 50 healthy Kunming mice were randomly divided into the following 5 groups: control group: only with surgery,not implement cerebral ischemia; ischemia-reperfusion groupⅠ( I / RⅠgroup),30 min of ischemia and reperfusion 1 h; ischemia-reperfusion Ⅱ groups: 60 min of ischemia and reperfusion 1 h group( I / RⅡgroup); ischemia-reperfusion Ⅲ group: 90 min of ischemia and reperfusion 1 h group( I / R Ⅲgroup); ischemia-reperfusion Ⅳgroup: 120 min of ischemia and reperfusion 1 h group( I / R Ⅳgroup). Then all mice were killed and the brain glands were taken for immunohistochemistry staining and image analysis by computer. Results ① The result of immunohistochemisty: the expression of Bcl-2 and Caspase-3 was pale brown in the brain neurons cells of mice of fasle operation group; the expression of Bcl-2 and Caspase-3 was brown yellow of I / R group; the expression of Bcl-2 and Caspase-3 was brown of I / R Ⅱgroup; compared with I / R Ⅱ group,the expression of Bcl-2 was reduced significantly in I / R Ⅲgroup and I / R Ⅳgroup. The expression of Caspase-3 was dark brown in the neurons cells of mice of I / R Ⅲ group; the expression of Caspase-3 was brown in I / R Ⅳ group. ②Comparison between the MOD of Bcl-2 in sham operation group and that of I / R Ⅱgroup was significant( P<0. 05). The expression of Bcl-2 was significantly enhanced than that of I / R Ⅰand Ⅲ group( P<0. 05),the difference of between the MOD of Bcl-2 in I / R Ⅱgroup and that of I / R Ⅳgroup was significant( P< 0. 05). The MOD of Caspase-3 in I / RⅠgroup,I / RⅡgroup,I / RⅢgroup and I / RⅣgroup were compared with that of sham operation group, there were significant differences( P<0. 05). the MOD of Caspase-3 in I / R Ⅱgroup and I / R Ⅲgroup were compared with that of I / RⅠ group,there were significant differences( P<0. 05); the difference of between the MOD of Caspase-3 in I / R Ⅱgroup and that of I / R Ⅲ group was significant( P<0. 05). The difference of Caspase-3 in I / R Ⅲ group and that of I / R Ⅳ group was significant( P<0. 05). Conclusions The expression of Caspase-3 is increased significantly than that of Bcl-2 in the impaired early ischemic neurons. However,as the ischemia time delay further,the expression of Bcl-2 is first reduced significantly,and laboratory basis is further provided for therapy early in acute cerebral ischemic diseases with Caspase inhibitors and Bcl-2 agonist.更多还原