【摘要】 目的探讨DcR3在预防博莱霉素大鼠模型发生肺纤维化中的作用。方法采用博莱霉素复制肺纤维化动物模型。分为正常对照组、博莱霉素组、博莱霉素+DcR3组,给药时间2 w,各组分别于2、4、6、8 w处死大鼠6只,收集肺泡灌洗液的细胞和上清检测细胞因子含量。左肺组织冻存留作羟脯氨酸检测;右肺组织固定用作组织染色。结果博莱霉素+DcR3组肺泡炎的程度表现为逐渐减轻的过程,炎症程度均低于BL组(P<0.05);BL+DcR3组肺纤维化程度无明显加重趋势,与对照组比较差异显著(P<0.05),与BL组比较有显著性差异(P<0.01)。结论 DcR3的早期应用,可减轻博莱霉素大鼠模型肺的的局部炎症,可能在一定程度上达到预防肺纤维化形成的目的。更多还原

【Abstract】 Objective To investigate the role of DcR3 in preventing bleomycin rat model of pulmonary fibrosis. Methods The bleomycin animal model of pulmonary fibrosis was established. The animal models were divided into normal control,bleomycin,bleomycin + DcR3 groups. The alveolar lavage fluid cells were cultured and supernatant,and the levels of cytokines were detected. Results The degree of alveolitis was reduced in Bleomycin+ DcR3 group,which was lower than that of BL group( P<0. 05); and the degree of lung fibrosis was not significantly increased trend in Bleomycin + DcR3 group,which had significant difference compared with that of BL and control groups( P<0. 05,P<0. 01). Conclusions DcR3 early application could reduce lung local inflammation in bleomycin rat model,achieve the purpose of prevention of pulmonary fibrosis in some extent.更多还原