【摘要】 目的观察褪黑素(MT)对阿尔茨海默病(AD)模型小鼠自主活动度和认知能力与钙离子浓度昼夜节律的调节作用及机制。方法 BALB/c小鼠右侧脑室注入β-淀粉样蛋白(Aβ1~42),制作AD小鼠模型并以MT干预,用ZZ小鼠自主活动测试仪检测自主活动度,Morris水迷宫测定认知能力,生化分析仪检测血清、脑浆钙离子浓度。结果 MT可改善AD小鼠自主活动、空间学习记忆能力的昼夜节律,对Aβ诱发的小鼠脑组织总钙含量增加均有显著的对抗作用。结论外源性MT可明显改善AD小鼠紊乱的生物近日节律,抑制细胞内Ca2+进入神经元发挥抗AD的作用。更多还原

【Abstract】 Objective To observe the regulation and mechanism of melatonin(MT) on the calcium ion concentration circadian rhythm of Alzheimer’s disease(AD) model mice’ autonomic activities and cognitive ability.Methods The animal model of AD was founded by injecting beta-amyloid(Abeta 1 ~ 42) into the lateral cerebral ventricle of BALB/c mice,then the AD model was intervented with MT.ZZ- fatigue rota-rod,Morris water maze,biochemical analysator were used to test the change of autonomic activity,cognitive ability,calcium ion concentration of hippocampal tissue.Results The circadian rhythm of AD model mice autonomic activities and spatial learning and memory ability had been ameliorated on the effect of MT,and MT significantly combated the rise of total calcium ion concentration in brain tissue of mice.Conclusions The exogenous MT remarkably improves the irregular circadian rhythm of AD model mice,and inhibits the intracellular calcium ion shifting to neuron to perform the regulatory effect of anti-AD.更多还原