【摘要】 反硝化厌氧甲烷氧化(DAMO)过程可以由一种称为Methylomirabilis oxyfera的DAMO细菌在有或者没有DAMO古菌下完成.已经报道的DAMO过程的菌群富集时间长(一般需要7~18月),且DAMO体系反硝化速率低.利用中空纤维膜生物反应器(HFMB)提高甲烷的传质来试图实现快速启动DAMO反应,结果发现HFMB在不到3个月时间内就表现出DAMO反应,其反硝化速率达到50 mg·L-1·d-1硝酸盐氮.二代测序显示,HFMB中微生物以Anaerolineaceae,Azospira,CL500-3占绝对优势,分别为39.08%,13.68%和11.54%,而DAMO细菌(Methylomirabilis)和与厌氧甲烷氧化有关的古菌Methanosarcina分别占0.02%和0.13%,因此推测在HFMB中DAMO过程是由一群新的菌群主导完成.更多还原

【Abstract】 The process of denitrifying anaerobic methane oxidation(DAMO)can be catalyzed by DAMO bacteria called Methylomirabilis oxyfera with or without the involvement of DAMO archaea.Enrichment of DAMO microbes often takes a long time(7~18 months)to show a faint denitrification function in reported DAMO process.This study aimed to fast enrich DAMO microbes in a hollow fiber membrane bioreactor(HFMB)as the special structure of hollow fiber can increase the mass transfer of methane.It is found that HFMB shows DAMO activity in a shorter period of time(<3months)and the denitrificationrate reaches 50mg·L-1·d-1 NO3--N.The next-generation DNA sequencing on the microbes in HFMB shows that Anaerolineaceae,Azospira,CL500-3are dominant with 39.08%,13.68% and 11.54% of the total microbes,respectively. Meanwhile, Methylomirabilis(DAMO bacteria)and Methanosarcina(DAMO archaea)are only 0.02% and 0.13%,respectively.It is speculated therefore that the DAMO process in HFMB may have been catalyzed by other microbes that are different from the known DAMO microbes.更多还原