【摘要】 目的:提高沙地柏多酚的纯化程度并考察其抗氧化活性。方法:采用Folin-Ciocalteu法测定沙地柏的多酚含量,经筛选用HPD-700大孔树脂纯化沙地柏多酚。通过对沙地柏多酚,1,1-二苯基-2-三硝基苯肼（DPPH）自由基、超氧阴离子、羟自由基的清除能力、总抗氧化能力及三价铁离子(Fe³⁺)还原能力的测定来评价其抗氧化能力。结果:经纯化后,沙地柏多酚纯度由0.53‰提高到9.95‰。抗氧化活性测定结果表明,沙地柏多酚对DPPH自由基的抑制率和Fe³⁺还原能力的维生素C当量质量浓度分别为9.4μg/ml、30.09μg/ml,对超氧阴离子和羟自由基的清除能力分别为151.83 U/ml、204.59 U/ml,总抗氧化能力为72.68 U/ml。结论:沙地柏多酚具有较强的抗氧化活性,值得进一步研究其药理作用。更多还原

【Abstract】 Objective:To purify polyphenols from Sabina vulgaris and to investigate its antioxidant properties.Methods:Polyphenols were purified from Sabina vulgaris Antoine with macroporous resin HPD-700,and the quantity of polyphenols was determined by Folin-Ciocalteu colorimetry.The antioxidant properties of polyphenols were evaluated by total antioxidant capacity（T-AOC） and its activeties of scavenging DPPH（1,1diphenyl-2-picry-hydrazyl） radicals,superoxide anion（O₂·^-）,hydroxyl free radicals（OH·） and ferric reducing antioxidant power（FRAP）.Results:After purification,the purity of polyphenols increased from 0.53%e to 9.95%e.The antioxidant properties study showed that its inhibition rate of scavenging DPPH radicals and FRAP was 151.83 U/ml and 204.59 U/ml.Its scavenging capacity for superoxide anion（O₂·） and hydroxyl free radicals（OH·） was 151.83 U/ml and 204.59U/ml.The total antioxidant capacity was 72.68 U/ml.Conclusion:Polyphenols from Sabina vulgaris Antoine have high antioxidant properties,suggesting that it worth further study of its pharmacological effects.更多还原