【摘要】 乳铁蛋白(LF)在抗炎和参与机体免疫调节方面具有重要作用。利用家蚕杆状病毒表达系统构建含人乳铁蛋白(hLF)基因的重组病毒Bm-hLF,接种蚕蛹表达重组人乳铁蛋白(rhLF),ELISA法检测rhLF的表达量,体外抑菌试验检测其抑菌活性,并建立小鼠溃疡性结肠炎(UC)模型,探究口服rhLF蚕蛹粉对UC的治疗效果。结果表明:rhLF在蚕蛹感染重组病毒120h后表达量最高达0.38mg/mL蚕蛹匀浆上清液;rhLF蚕蛹粉能抑制E.coli TG1的生长;口服rhLF蚕蛹粉可明显降低UC小鼠的疾病活动指数(DAI)、髓过氧化物酶(MPO)含量,减轻结肠组织炎症损伤,为今后开发一种新型、廉价的UC口服药物提供药效学实验参考。更多还原

【Abstract】 Lactoferrin(LF)plays an important role in anti-inflammation and participation in body immunity moderation.This paper constructs recombinant virus Bm-hLF containing human lactoferrin(hLF)through silkworm baculovirus expression system,inoculates silkworm chrysalis expression recombinant human lactoferrin(rhLF),tests the expression quantity of rhLF with ELISA method,detects its bacteriostatic activity through antibacterial experiment in vitro,establishes mouse ulcerative colitis(UC)model and explores the therapeutic effect of oral administration of rhLF silkworm chrysalis meal on UC.The result shows that the maximum expression quantity of rhLF can be 0.38mg/mL silkworm chrysalis homogenate supernatant 120 hafter silkworm chrysalis is infected with recombinant virus.rhLF silkworm chrysalis meal can inhibit the growth of E.coli TG1.Oral administration of rhLF silkworm chrysalis meal can greatly reduce the disease activity index(DAI)and myeloperoxidase(MPO)content of UC mice and reduce the damage of colon tissue inflammation.This study provides pharmacodynamic experimental reference for further development of a new and cheap UC oral drug.更多还原