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杜鹃花类菌根菌株对桃叶杜鹃幼苗硝酸还原酶活性和氮的影响

Nitrate reductase activity and N absorption of Rhododendron annae seedlings with ericoid mycorrhiza inoculation

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【作者】 欧静刘仁阳张仁嫒谌端玉王丽娟陈训

【Author】 OU Jing;LIU Renyang;ZHANG Ren’ai;CHEN Duanyu;WANG Lijuan;CHEN Xun;Forestry College,Guizhou University;Guizhou Academy of Science;

【机构】 贵州大学林学院贵州省科学院

【摘要】 研究12株杜鹃花类菌根(ericoid mycorrhiza,ERM)菌株对2年生桃叶杜鹃Rhododendron annae幼苗生长及矿质元素氮积累的影响。结果表明:接种ERM真菌的植株根系均被有效地感染,不同菌株促生效应显著,接种ERM真菌显著增加幼苗地上、地下部分及总生物量。与对照相比,ERM菌株接种后,显著提高了接种幼苗氮质量分数和硝酸还原酶(NR)活性,且各处理差异极显著(P<0.01)。接种苗根部氮质量分数提高了2.0%~40.3%,叶部提高了2.8%~50.6%。接种苗根部硝酸还原酶活性除菌株TY41外,其余处理高于对照0.9%~29.3%,叶部除菌株TY41和菌株TY24外,高于对照6.5%~43.9%。不同器官硝酸还原酶活性表现为根系大于叶片。

【Abstract】 To measure growth and N accumulation in two-year-old Rhododendron annae seedlings,twelve ericoid mycorrhiza(ERM) fungal strain isolates were used for inoculation.The test design set 13 treatments(including ck),each disposed 5 trees,3 times repeated.The inoculated method was soil inoculation.Results showed that ERM fungi colonized the seedling roots improving biomass of seedling aboveground and belowground parts.Compared to uninoculated controls,inoculation with different ERM strains showed a highly significant increase(P <0.01) for N content in the leaves and roots of seedlings as well as nitrate reductase(NR) activity.Root N content increased 2.0%-40.3%,and foliar N increased 2.8%-50.6%.Except for TY14,root NR activity was higher than the control(0.9%-29.3%),and except for TY41 and TY24,foliar NR activity was higher than the control 6.5%-43.9%.NR activity in different organs showed root>leaf.ERM fungal strain promoted the growth,NR activity and N content of R.annae seedlings,which meant to promote seedling nitrogen absorption and utilization.Effects of mycorrhiza varied by different ERM fungi strain.

【基金】 贵州省农业科技攻关项目(黔科合NY字[2011]3076号);贵州大学引进人才科研项目[贵大人基合字(2013)07号]
  • 【文献出处】 浙江农林大学学报 ,Journal of Zhejiang A & F University , 编辑部邮箱 ,2014年06期
  • 【分类号】S685.21
  • 【被引频次】13
  • 【下载频次】201
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