【摘要】 目的 观察脑缺血-再灌注对血清NO和CO的影响及颅痛定的干预。方法随机抽样2011-01~2012-12收治的心肺复苏术后35例患者,随机分为治疗组20例,对照组15例。两组患者心肺复苏后立即给予一般性治疗,治疗组起病后即加用颅痛定60 mg,每日3次,持续3 d用药。于心肺复苏术后6、24、48、72 h抽血测定NO、CO浓度。硝酸还原酶法测定血清NO浓度,可见分光光度计法测定CO浓度。结果 人脑缺血-再灌注6、24、48、72 h后外周静脉血中NO浓度增加,其中脑缺血<5 min再灌注48 h达到高峰;人脑缺血-再灌注6 h后CO浓度最大,之后回落,其中脑缺血<5 min再灌注24 h达到高峰;颅痛定降低脑缺血-再灌注6~24 h CO浓度,增加NO浓度,而在缺血-再灌注24~72 h降低NO浓度。结论 颅痛定可能通过调控机体内NO和CO浓度,发挥抗脑缺血-再灌注损伤作用。更多还原

【Abstract】 Objective To observe the effects of L- tetrahydropalmatine(L- THP) on nitric oxide(NO) and carbon monoxide(CO) concentration in plasma during cerebral ischemia reperfusion.Methods Cardiopulmonary resuscitation(CPR) of 35 cases of postoperative patients were divided into 2groups in random;treatment group(n =20)and control group(n = 15).Two groups of patients after CPR immediately to give general treatment.Treatment group after the onset of the cranial pain.Nitrate reductive enzymatic method was used to detect the serum content of NO;visible spectrophotometer method was used to detect the serum content of CO.Results NO content was elevated at 6,24,48,72hour,and peaked at 48 hour when cerebral ischemia occurred within 5 minutes;CO content peaked at 6hour,while peaked at 24 hour when cerebral ischemia occurred within 5 minutes;L- THP significantly increased NO concentrations and reduced CO concentrations after cerebral ischemia reperfusion from 6 to24 hour,while reduced NO concentrations after cerebral ischemia reperfusion from 24 to 72 hour.Conclusion L- THP probably has a good effect on cerebral ischemia- reperfusion injury by regulating the NO and CO contents.更多还原