The detection and clinical study of the levels of transcription of receptors in the lower respiratory tract of infants following infection with respiratory syncytial virus
Objectives To use respiratory syncytial virus to experimentally infect lower respiratory tract cells from infants and to ascertain levels of transcription of receptors at different times in order to prevent and treat these infections.Methods Cells from the lower respiratory tract of infants were cultured and divided into two groups,one infected with respiratory syncytial virus and a control group.Total RNA was extracted and qualitatively and quantitatively analyzed.It was then subjected to RT-PCR and gel el...