Objective To prepare an antigen found in Trichomonas vaginalis trophozoites and to immunize rabbits with the trophozoite antigen in order to detect its immunogenicity. Methods Clinical isolates of T.vaginalis were seeded in TYM medium and maintained as axenic cultures to produce the trophozoite antigen.Rabbits were immunized by subcutaneous injections of the trophozoite antigen in the skin of the back.Rabbits were either given a booster(2weeks later)or they were not immunized.Seven days later,blood was coll...
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