Objective o examine the sensitivity and specificity of a PCR-based pooling strategy at detecting malaria parasites at different densities in order to better monitor malaria after malaria elimination. Methods Two sensitive PCR techniques were used to detect malaria parasites at 8different densities in a single specimen,in a pool of 5specimens,in apool of 25 specimens,in a pool of 50 specimens,and in a pool of 100 specimens in order to determine the sensitivity and specificity of the two methods.These 8densit...
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