To establish double-antibody sandwich ELISA to detect Giardia canis antigen(α-12Giardin)in dog feces. Methods The monoclonal antibody 4C9 was labelled with HRP.Fecal samples were filtered and subjected to ultrasound.The monoclonal antibody 3H10 was used as a capture antibody,and the HRP-labeled monoclonal antibody 4C9 was used as a detection antibody;5%skimmed milk powder was used as a blocking agent.Once the color of the substrate changed,the absorbance at 490nm(A490)was measured.The optimal concentration ...
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