【摘要】 目的检测胃癌患者血管内皮因子C(VEGF-C)蛋白及m RNA在胃癌组织中的表达情况,探讨其与胃癌淋巴结微转移的关系。方法应用免疫组织化学法及RT-PCR法检测80例胃癌组织、癌旁组织及正常组织中的VEGF-C蛋白及m RNA表达情况;免疫组化法检测淋巴结微转移情况,比较有无淋巴结微转移的VEGF-C蛋白和m RNA的表达差异,明确存在的关联性。结果VEGF-C蛋白及m RNA两者在胃癌组织中明显高于癌旁组织和正常组织(P<0.05)。结论胃癌组织中的VEGF-C蛋白及m RNA与胃癌淋巴结微转移相关,基因检测优于蛋白,可作为评价胃癌患者是否存在淋巴结微转移的优选指标。更多还原

【Abstract】 Objective To explore the expression of vascular endothelial growth factor-C(VEGF-C) protein and m RNA in gastric cancer and its relationship with lymph node micrometastases. Methods Immunohistochemistry and RT PCR were used to detect the expression of VEGF-C protein and m RNA in gastric carcinoma tissue, adjacent tissue and normal tissue from 80 patients who suffered from gastric carcinoma. The expression level of VEGF-C protein and m RNA were compared between tissues with positive or negative lymph node micrometastases. Results The positive expression rates of VEGF-C protein and m RNA in gastric cancer tissue were significantly higher than those in adjacent tissue and normal tissue(P< 0.05), which had no relationship with the patients’ age, sex, tumor size, location and distant metastasis. The expression of VEGF-C was positively correlated with lymph node metastasis(P < 0.01), and that of VEGF-C m RNA with micrometastasis was closer than that of protein. Conclusions VEGF-C protein and m RNA in gastric cancer tissue is correlated with lymph node micrometastases, and detection of VEGF-C m RNA is prior to detection of protein. VEGF-C m RNA can be serving as a more accurate indicator of lymph node micrometastases in the gastric cancer patients.更多还原