【摘要】 [目的]探讨体外DNA结合抑制因子(inhibitor of DNA bindingor differentiation,Id)的表达对卵巢上皮癌SKOV3对顺铂化疗敏感性的影响。[方法]构建靶向Id1基因siRNA慢病毒载体并转染SKOV3细胞,筛选出稳定转染的SKOV3细胞克隆。实验分为4组:实验组(Cell+LvshRNA-Id1)、阴性对照组(Cell+Lv-shRNA-NC)、病毒对照组(Cell+Lv-control)与空白对照组(Cell group)。CCK8检测各组SKOV3细胞不同时间增殖活性以及暴露于不同浓度顺铂(0.1、0.2、0.5、1、2、5、10μg/ml)48h后顺铂对各组SKOV3细胞的半数抑制浓度,分析沉默Id1基因后卵巢癌细胞对顺铂化疗敏感性的影响。[结果]三组对照组细胞增殖活性随着时间增加显著,而实验组则增加缓慢。在48h和72h时,实验组细胞增殖活性值分别为0.449±0.072μg/ml、0.885±0.232μg/ml,与三组对照组比较差异均有统计学意义(P均<0.05);实验组顺铂对SKOV3细胞的半数抑制浓度为1.5±0.71μg/ml,明显低于对照组(P<0.01)。[结论]抑制Id1基因表达可以增加顺铂对卵巢癌细胞的生长抑制作用,为临床进一步提高卵巢癌的疗效提供了研究基础。更多还原

【Abstract】 [Purpose] To investigate the effect of inhibitor of DNA binding or differentiation 1( Id1) on the chemosensitivity of ovarian epithelial carcinoma SKOV3 cells to cisplatin in vitro.[Methods] The lentiviral vector of siRNA targeting Id1 gene was constructed and transfected into SKOV3 cells,stably transfected SKOV3 cell was selected and cloned. The experiment was divided into four groups:Cell+Lv-shRNA-ID1、Cell+Lv-shRNA-NC、Cell +Lv-control and Blank cell group. The proliferations of SKOV3 cells at different times were detected by CCK8. SKOV3 cells were exposed to different concentrations of cisplatin(0.1、0.2、0.5、1、2、5、10μg/ml) for 48 hours in each group and half inhibitory concentrations of cisplatin to SKOV3 cells were detected by CCK8. The effect of targeting Id1 gene on the chemosensitivity in ovarian cancer cells to cisplatin was analyzed. [Results]Cell proliferations in three control groups increased significantly over time,cell proliferations in the Cell +LvshRNA-ID1 group increased slowly. Cell proliferations were 0.449±0.072μg/ml at 48 h and 0.885±0.232μg/ml at72 h in the Cell+Lv-shRNA-ID1 group,the differences were statistically significant compared with three control groups(P all <0.05). Half inhibitory concentration of cisplatin to SKOV3 cells in the Cell+Lv-shRNA-ID1 group was 1.5±0.71μg/ml which was significantly lower than that in three control groups(P<0.01) with no significant difference among the three control groups. [Conclusion] Silence of Id1 expression increases growth inhibition effect of cisplatin to ovarian cancer cells. It provides the research foundation for further improving the clinical efficacy for ovarian cancer.更多还原