【摘要】 目的:探讨不同分期(T2~T4)的尿路膀胱上皮癌(urothelial bladder carcinoma,UBC)中人类表皮生长因子受体-2(HER2)蛋白表达和基因扩增状态。方法:将49例不同分期的膀胱癌患者的肿瘤组织进行HER2免疫组织化学染色,以及HER2基因的双色荧光原位杂交(FISH)检测。结果:膀胱癌患者男性居多。而且分期越高,HER2染色阳性率越高。但所有患者均未出现基因扩增,有12例患者出现17号染色体多倍体。分期越高,多倍体发生越多。结论:膀胱癌患者HER2蛋白增高不是由于基因扩增所致,其它的转录和后转录机制参与并调节了蛋白的表达。更多还原

【Abstract】 Objective: To explore the expression and gene amplification status of human epidermal growth factor receptor-2(HER2) in the different stages of invasive urothelial bladder carcinoma. Methods: Tumor tissues from 49 patients with different stages of invasive urothelial bladder carcinoma were tested by immunohistochemical staining for HER2 and HER2 gene fluorescence in situ hybridization. Results: The number of male patients was higher than that of females. The positive rate of HER2 protein expression was higher in the patients with the higher stage of invasive urothelial bladder carcinoma. However, no gene amplification was observed in all patients. Twelve patients had ployploid chromosome 17. More ployploids were observed in the patients with the higher stage of invasive urothelial bladder carcinoma. Conclusion: The increase in the protein expression of HER2 in the invasive urothelial bladder carcinoma patients was not caused by gene amplification. Other transcriptional and post-transcriptional mechanisms were probably involved in the regulation of the HER2 protein.更多还原