【摘要】 目的建立同时测定不同产地的益智的不同部位中5-羟甲基糠醛(1),teuhetenone A(2),杨芽黄素(3)和诺卡酮(4)含量的超高效液相色谱法(UPLC)。方法采用ACQUITY UPLC,HSS T3色谱柱(2.1 mm×100 mm,1.8μm),流动相为乙腈-0.1%的甲酸水溶液梯度洗脱,流速为0.5 mL·min-1,检测波长采用255 nm。结果化合物1,2,3和4分别在1.223~24.46,2.016~40.32,1.875~37.50和16.78~335.6μg·mL-1线性关系良好,平均回收率分别为99.5%,101.5%,100.9%和101.2%。结论化合物2和4主要分布于种子中,而化合物1和3均分布于种子和果壳中;该方法快速、简单、重复性好,可以为益智的鉴别和质量控制提供依据。更多还原

【Abstract】 OBJECTIVE To establish a UPLC method for simultaneous determination of 5-hydroxymethyl furfural( 1),teuhetenone A(2),tectochrysin(3),and nootkatone(4)in Alpinia oxyphylla Miq,and to compare the contents of the four components in different parts of this medicinal materials from different places. METHODS The UPLC method was established on an HSS T3 Column(2. 1 mm × 100 mm,1. 8 μm). The mobile phase consisted of water containing 0. 1% formic acid and acetonitrile in gradient elution mode at the flow rate of 0. 5 mL·min- 1and the detection wavelength was set at 255 nm. RESULTS The standard curves of compounds 1,2,3,and 4 showed good linearity in the ranges of 1. 223- 24. 46,2. 016- 40. 32,1. 875- 37. 50 and 16. 78- 335. 6 μg·mL- 1with the corresponding average recoveries of 99. 5%,101. 5%,100. 9% and 101. 2%,respectively. CONCLUSION The method is precise and highly reproducible,which can be used to simultaneously determine the antioxidant components including 5-hydroxymethyl furfural,teuhetenone A,tectochrysin,and nootkatone in Alpinia oxyphylla Miq; compounds 2 and 4 are mainly extracted from the seeds,while compounds 1 and 3 come from the seeds and putamens equally. There are significant differences among the samples from different production areas.更多还原