【摘要】 目的研究雷山石杉的化学成分。方法使用制备薄层、硅胶柱色谱、Sephadex LH-20及半制备高效液相色谱法等手段进行分离、纯化,结合文献资料、理化性质、1H-NMR及13C-NMR数据进行结构鉴定。结果分离得到10个化合物,分别鉴定为(15R)-12,16-epoxy-11,14-dihydroxy-8,11,13-abietatrien-7-one(Ⅰ),21-epi-serratenediol-3,21-acetate(Ⅱ),serratene-diol-3-acetate(Ⅲ),21-epi-serratenediol-3-acetate(Ⅳ),21α-hydroxy-serrat-14-en-3β-ol(Ⅴ),21β-hydroxy-serrat-14-en-3β-ol(Ⅵ),石杉碱甲(huperzine A,Ⅶ),蔗糖(Ⅷ),β-谷甾醇(Ⅸ),胡萝卜苷(Ⅹ)。结论化合物Ⅰ~Ⅹ均首次从该植物中分离到。更多还原

【Abstract】 OBJECTIVE To study the chemical constituents in the whole plant of Huperzia leishangensis. METHODS Compounds were isolated and purified by Prep-TLC,silica gel chromatography,Sephadex LH- 20 and preparative high performance liquid chromatography,and their structures were identified on the basis of comprehensive analysis of their physical and chemical properties, NMR data and literatures. RESULTS Ten compounds were isolated and elucidated as(15R)-12,16-epoxy-11,14-dihydroxy-8,11, 13-abietatrien-7-one( Ⅰ),21-epi-serratenediol-3,21-acetate( Ⅱ),serratenediol-3-acetate( Ⅲ),21-epi-serratenediol-3-acetate( Ⅳ), 21α-hydroxy-serrat-14-en-3β-ol(Ⅴ),21β-hydroxy-serrat-14-en-3β-ol( Ⅵ),huperzine A( Ⅶ),sucrose( Ⅷ),β-sitosterol( Ⅸ) and β-daucosterol(Ⅹ). CONCLUSION Compound Ⅰ- Ⅹ were for the first time isolated from H. leishangensis.更多还原