【摘要】 目的研究8类聚山梨酯80的化学组分刺激肥大细胞RBL-2H3脱颗粒的作用,阐明聚山梨酯80中引发类过敏反应的物质基础。方法以不同浓度的8类聚山梨酯80的化学组分处理RBL-2H3细胞,测定β-氨基己糖苷酶释放率,并通过四甲基偶氮唑蓝法研究受试物对RBL-2H3细胞的毒性作用。结果聚氧乙烯山梨醇酐一油酸酯、聚氧乙烯山梨醇酐二油酸酯和聚氧乙烯异山梨醇一油酸酯均能直接刺激RBL-2H3细胞脱颗粒,呈浓度依赖性;聚氧乙烯山梨醇酐和聚氧乙烯异山梨醇在本研究的浓度范围内均不能直接刺激RBL-2H3细胞脱颗粒;聚氧乙烯山梨醇酐三油酸酯、聚氧乙烯山梨醇酐四油酸酯、聚氧乙烯山梨醇酐二油酸酯刺激RBL-2H3细胞脱颗粒的作用较弱,并呈浓度依赖性。聚氧乙烯山梨醇酐一油酸酯、聚氧乙烯山梨醇酐二油酸酯和聚氧乙烯异山梨醇一油酸酯有明显的细胞毒性;聚氧乙烯山梨醇酐和聚氧乙烯异山梨醇无细胞毒性;聚氧乙烯山梨醇酐三油酸酯、聚氧乙烯山梨醇酐四油酸酯、聚氧乙烯山梨醇酐二油酸酯显示低的细胞毒性,并呈浓度依赖性。结论聚氧乙烯异山梨醇一油酸酯是聚山梨酯80最主要的毒性物质来源,必须加以限量控制。更多还原

【Abstract】 OBJECTIVE To study directive effects of eight types of the chemical composition of polysorbate 80 on the degranulation in the RBL-2H3 rat mast cell line and to illuminate the material basis for its pseudoallergy. METHODS RBL-2H3 cells were cultured and treated with varying dosage of eight types of the chemical composition of polysorbate 80. And then the amount of the released β-hexosaminidase was detected. MTT assay was used to determine the cytotoxicity of eight types of the chemical composition of polysorbate 80 which causing RBL-2H3 cells degranulation. RESULTS PSM,PSD and PIM induced RBL-2H3 cells’ degranulation in a concentrationdependent manner. Degranulation was not detected in cells treated with PS and PI. PSTri,PSTetra and PSD weakly induced the degranulation of RBL-2H3 cells in a concentration-dependent manner. PSM,PSD and PIM showed cytotoxicity with concentration-dependent manner,while PS and PI had no cytotoxicity. PSTri,PSTetra and PSD showed low toxicity cytotoxicity in a concentration-dependent manner. CONCLUSION PIM is a major source of toxic substances polysorbate 80,the limit of which must be controlled.更多还原