【摘要】 目的 分析雷帕霉素靶蛋白(mTOR)、70 ku核糖体蛋白S6激酶(P70S6K)的表达水平与胃癌常见临床病理因素的关系。方法 采用逆转录聚合酶链反应技术,蛋白质印迹技术(Western-blot)检测32例胃癌患者癌组织、癌旁胃组织以及22例非胃癌患者胃组织中磷酸化雷帕霉素靶蛋白(mTOR)及磷酸化70 ku核酸体蛋白S6激酶(P70S6)K mRNA和蛋白水平的表达情况。结果 p-mTOR mRNA在胃癌组织中的表达水平(0.61±0.11)明显高于癌旁胃组织(0.43±0.10)及非胃癌胃组织(0.39±0.07),p-P70S6KmRNA在胃癌组织中的表达水平(0.63±0.13)亦明显高于癌旁胃组织(0.45±0.14)及非胃癌胃组织(0.44±0.15),差异均有统计学意义(均P<0.05);mTOR mRNA和P70S6K mRNA在胃癌组织中的表达呈正相关(r=0.521,P=0.033);Western-blot检测结果也证实p-mTOR及p-P70S6K在胃癌组织中的表达高于癌旁胃组织及非胃癌胃组织;mTOR及P70S6K mRNA在胃癌组织中的表达水平与病理分期、淋巴结转移等明显相关,而与肿瘤直径、性别等无明显关系。结论 mTOR/P70S6K信号通路在胃癌组织中过度表达可能是胃癌发生的重要机制之一。更多还原

【Abstract】 Objective To evaluate the mammalian target of rapamycin(mTOR)/70 ku ribosomal protein S6 kinase(P70S6K) in gastric carcinoma.Methods Reverse transcription-polymerase chain reaction and Western-blot were used to determine the expression of mTOR/P70S6 K signaling pathway in cancerous and paraeancerous tissues in 32 cases and 22 normal healthy tissues.Results The expression level of mTOR mRNA was markedly higher in the gastric carcinoma tissues(0.61 ±0.11) than in the paracaneerous tissues(0.43 ±0.10)and normal stomach tissues(0.39 ±0.07);the expression of P70S6 K mRNA was also higher in the gastric carcinoma tissues(0.63 ±0.13) than in the paracaneerous tissues(0.45 ±0.14) and normal stomach tissues(0.44 ±0.15),with a significant difference among groups(P < 0.05).There was a positive correlation between mTOR mRNA and P70S6 K mRNA expression in gastric carcinoma tissues(r = 0.521,P= 0.033).Compared to those in other two groups,the protein expression of mTOR,P70S6 K in gastric carcinoma were significantly increased.The expression of mTOR/P70S6 K signaling pathway in the gastric carcinom tissues was significantly correlated with the pathological grading and lymph node metastasis,but not with the diameter and gender.Conclusions The mTOR/P70S6 K signaling pathway is specifically activated in gastric carcinom tissues.The mTOR/P70S6 K signaling pathway might play an important role in the pathogenesis of gastric carcinoma.更多还原