【摘要】 目的 探讨血清25-羟维生素D₃[25-（OH）D₃]及总水平与婴幼儿反复喘息的关系。方法 分别采用酶联免疫吸附测定、免疫比浊法,检测反复喘息的婴幼儿60例（反复喘息婴幼儿组）及正常对照儿童60例（对照组）血清中25-（OH）D₃、TIgE含量。采用全自动血细胞分析仪检测2组外周血嗜酸性粒细胞百分比（EOS%）,并做比较。结果 反复喘息婴幼儿组血清25-（OH）D₃含量明显低于对照组,2组比较差异有统计学意义[（18±6）μg/L比（38±8）μg/L,t=15.81,P=0.000];反复喘息婴幼儿组血清TIgE含量、EOS%明显高于对照组,2组比较差异有统计学意义[（122±18）IU/ml比（51±10）IU/ml,t=25.84,P=0.000;（3.02±1.82）%比（0.72±0.27）%,t=9.73,P=0.000];反复喘息婴幼儿组血清25-（OH）D₃含量与TIgE含量存在负相关（r=-0.783,P=0.000）,对照组血清25-（OH）D₃含量与TIgE含量无相关性（r=-0.013,P=0.924）。2组血清25-（OH）D₃含量与EOS%无相关性（r=-0.084,P=0.523;r=-0.137,P=0.298）。结论 血清25-（OH）D₃的低下可能为婴幼儿反复喘息的高危因素;反复喘息的婴幼儿组血清25-（OH）D₃与TIgE有明显负相关性,表明婴幼儿反复喘息与变态反应密切相关。补充维生素D₃可能为临床预防婴幼儿反复喘息提供新思路。更多还原

【Abstract】 Objective To study the changes and clinical significance of serum levels of 25 hydroxy vitamin D,[25-（OH）D₃]and total IgE and peripheral blood eosinophilia cell percentage（EOS%）in infants with recurrent wheezing.Methods Sixty infants with recurrent wheezing and sixty healthy children were enrolled.Enzyme linked immunosorbentassay was used to detect the levels of serum 25-（OH）D,and immunoturbidimetry was used to detect the levels of total IgE.Automatic blood cell analyzer was used to detect EOS%of the two groups.Results Serum 25-（OH）D₃ levels decreased significantly in infants with recurrent wheezing group,compared with the normal group[（18 ±6） μg/L vs（38 ±8） μg/L]（t =15.81,P =0.000）.In contrast,serum total IgE levels increased significantly in infants with recurrent wheezing group,compared with the normal group[（122 ± 18）IU/ml vs（51 ± 10）IU/ml,（3.02±1.82）%vs（0.72 ±0.27）%]（t =25.84,P=0.000）.EOS%increased significantly in the infants with recurrent wheezing group compared with the normal group（t =9.73,P =0.000）.Serum 25-（OH）D₃ levels were negatively correlated with serum total IgE level in the infants with recurrent wheezing group（r =-0.768,P =0.000）.There was no correlation between serum 25-（OH）D₃ levels and serum total IgE levels in the normal group（r=-0.013,P =0.924）.Serum 25-（OH）D₃ levels had no correlation with EOS%in both groups（r=-0.084,P=0.523;r=-0.137,P =0.298）.Conclusions Low serum 25-（OH）D₃ may be the risk factor of recurrent wheezing in infants.Serum 25-（OH）D₃ levels are negatively correlated with serum total IgE levels in the infants with recurrent wheezing group;recurrent wheezing in the infants is closely related to allergic reaction.The supplement of vitamin D,may provide new ideas for clinical prevention of infant recurrent wheezing.更多还原