【摘要】 目的 探讨脂肪因子Apelin-13对3T3-L1脂肪细胞水通道蛋白7（AQP7）基因表达的影响。方法 体外培养3T3-L1脂肪细胞,以油红O染色鉴定为成熟脂肪细胞后,分为阴性对照组（无干预）,不同浓度(10^-9、10^-8、10^-7、10^-6nmol/L)Apelin-13干预组（均干预24 h）,阳性对照组(10^-5nmol/L罗格列酮干预24 h)和10^-7nmol/L Apelin-13干预不同时间（0、12、24、36、48 h）组。用反转录.聚合酶链反应检测各组细胞AQP7 mRNA表达水平。结果 阴性对照组、10^-9、10^-8、10^-7、10^-6nmol/L Apelin-13和阳性对照组AQP7表达水平分别为（0.22±0.02）、（0.29±0.07）、（0.36±0.05）、（0.43±0.05）、（0.31±0.06）、（0.32+0.08）,阳性对照组与阴性对照组之间差异有统计学意义（P<0.05）;与阴性对照组比较,10^-8、10^-7nmol/L Apelin-13能明显刺激AQP7 mRNA表达（P<0.05）;10^-8、10^-7nmol/L Apelin-13组与阳性对照组比较,10^-7nmol/L Apelin-13能明显刺激AQP7 mRNA表达（P<0.05）;10^-8、10^-7nmol/L Apelin-13组间差异无统计学意义。在时间组中,10^-7nmol/L Apelin-13的0、12、24、36、48 h各组灰度比值分别为（0.27±0.09）、（0.43±0.07）、（0.55±0.10）、（0.42±0.08）、（0.33±0.09）,12、24、36 h组AQP7 mRNA表达较0 h组能明显刺激AQP7 mRNA表达（P<0.05）,作用24 h表达最高;但12、24、36 h 3组之间AQPTmRNA表达差异无统计学意义。结论 Apelin-13在一定程度上能增加3T3-L1脂肪细胞AQP7 mRNA表达的水平,并分别以10^-7nmol/L和24 h为最佳作用浓度和时间。更多还原

【Abstract】 Objective To investigate a dependent manner of adipocykine Apelin-13 on Aquaporin（AQP7） gene expression in 3T3-L1 adipocyte.Methods The fully differentiated 3T3-L1 adipocytes identified by red oil 0 staining were divided into groups including negative control group,Apelin-13 treated groups with concentrations of 10^-9,10^-8,10^-7,10^-6 nmol/L for 24 h and with concentration of 10^-7 nmol/L for 0,12,24,36 and48 h and positive control group treated with rosiglitazone for 24 h with the concentration of 10^-5 nmol/L.The expression of AQP7 mRNA was measured according to the reverse trans cription-polymerase reaction.Results The expressions of AQP7 mRNA in the negative control group,Apelin-13 treated groups with concentration of 10^-9,10^-8,10^-7,10^-6 nmol/L and positive control group were（0.22 ±0.02）,（0.29 ±0.07）,（0.36 ± 0.05）,（0.43 ±0.05）,（0.31 ±0.06）and（0.32 ±0.08） respectively.There were significant differences between positive control group and negative control group（P < 0.05）;compared with negative control group,10^-8,10^-7nmol/L Apelin-13 significantly stimulated AQP7 mRNA expression（P < 0.05）;10^-8,10^-7 nmol/L Apelin-13group compared with positive control group,10^-7 nmol/L Apelin-13 significantly stimulated AQP7 mRNA expression（P<0.05）;there showed no statistical difference between 10^-8 and 10^-7 nmol/L Apelin-13 group.In the time group,the garg-scale ratio of 0,12,24,36,48 h of 10^-7nmol/L Apelin-13 were（0.27 ±0.09）,（0.43 ±0.07）,（0.55 ±0.10）,（0.42 ±0.08）,（0.33 ±0.09） respectively.12,24,36 h group compared with 0 h group significantly stimulated AQP7 mRNA expression（P<0.05）,and the highest expression at 24 h;but there were no significant differences between the 12,24,36 h group.Conclusion To some extent,Apelin-13 increases AQP7 mRNA expression in 3T3-L1 adipocytes with the excellence concentration of 10^-7 nmol/L treatment for 24 h.更多还原