【摘要】 建立怀牛膝中3种甾酮类成分含量测定方法,比较不同产地牛膝的质量差异。色谱柱为Venusil XBP C18柱,流动相为0.1%甲酸-乙腈(84∶16),检测波长为250 nm。在所建立的方法中,β-蜕皮甾酮、R-牛膝甾酮和S-牛膝甾酮与杂质分离良好,线性范围分别为3.07~1 470.00μg/mL(r=0.999 4,n=6),0.52~246.00μg/mL(r=0.999 7,n=6),0.47~225.00μg/mL(r=0.999 8,n=6),平均回收率分别为101.4%、101.1%和101.1%。本方法适用于同时测定怀牛膝中β-蜕皮甾酮、R-牛膝甾酮及S-牛膝甾酮的含量。更多还原

【Abstract】 An HPLC method was established for the simultaneous determination of β-ecdysterone,R-inokosterone and S-inokosterone in the Achyranthis bidentatae Radix to compare the quality from different habitats. Three phytoecdysones were separated with a Venusil XBP C18column by isocratic elution using 0. 1% formic acid in water and acetonitrile( 84 ∶ 16) as the mobile phase,while the UV detector wavelength was set at 250 nm. Calibration curves of β-ecdysterone,R-inokosterone and S-inokosterone were linear over the range of 3. 07-1 470. 00, 0. 52-246. 00,and 0. 47-225. 00 μg / mL,respectively. Average recoveries were 101. 4%,101. 1% and 101. 1%, respectively. The established method will be helpful in the quality control of Achyranthis bidentatae Radix.更多还原