【摘要】 为研究半边旗中二萜类化合物5F在体内对乳腺癌生长和转移的影响及其作用机制,以MDA-MB-231乳腺癌原位移植模型观察5F抗乳腺癌的效应。在治疗的同时,观察5F对裸鼠的不良反应。以RT-PCR、Western blot法检测5F对乳腺肿瘤组织血管内皮生长因子(VEGF)、激酶嵌入结构域受体(KDR)mRNA和蛋白表达的影响。结果显示,5F通过减少原发肿瘤体积和肺转移结节数目,显著抑制MDA-MB-231乳腺癌移植瘤的生长和肺转移。这种抑制转移的作用不依赖于其对原发肿瘤的生长抑制作用。5F对裸鼠的肝肾功能无明显的影响。5F抗MDA-MB-231乳腺癌作用机制与5F下调肿瘤组织VEGF、KDR mRNA和蛋白表达水平有关。更多还原

【Abstract】 The aim of this study was to investigate the effect and its possible mechanism of the diterperoid compound 5F isolated from Pteris semipinnata L. on breast cancer growth and metastasis in MDA-MB-231 orthotopic breast tumor xenograft model. The potential side-effects were monitored in addition to its therapeutic effect. RT-PCR and Western blot were applied to detect the expression levels of vascular endothelial growth factor( VEGF),kinase domain insert containing receptor( KDR) in breast cancer tissue. Results showed that 5F significantly suppressed breast cancer growth and metastasis in nude mice by reducing the volume of primate tumor and the number of metastatic nodules in lung. Furthermore,5F suppressed metastases independent of its inhibitory effect on primary tumor growth. 5F treatment exhibited no toxic effect on liver or kidney in nude mice. 5F can suppress growth and metastasis of MDA-MB-231 breast cancer xenograft. Its mechanism may be involved in the deduction of the expression levels of VEGF and its main receptor KDR in tumor tissue.更多还原