【摘要】 目的:探讨氯化钠(Na Cl)浓度对黄芩幼苗生长情况及生理指标的影响,为黄芩药材的大田生长提供依据。方法:采用盆栽方式,待黄芩幼苗长至8~11 cm时,用1/2 Hoagland营养液兑加不同剂量的Na Cl,模拟盐胁迫环境,培养3 d后统计幼苗成活率,并采用紫外-可见分光光度法测定叶绿素和类胡萝卜素的含量,用硫代巴比妥酸法测定丙二醛的含量,用电导法定膜透性。结果:随着Na Cl浓度的增大,黄芩幼苗成活率、叶绿素和类胡萝卜素的含量均呈先升后降的趋势,丙二醛含量和相对电导率呈逐渐升高的趋势;其中,Na Cl浓度为0.4%时,黄芩幼苗成活率、叶绿素和类胡萝卜素的含量均达到峰值。结论:黄芩幼苗的临界耐盐浓度为0.4%,盐浓度<0.4%时对黄芩幼苗的生长无明显影响,>0.4%时盐害作用较严重。更多还原

【Abstract】 OBJECTIVE:To investigate the effect of Na Cl concentration on seedling growth and physiological indexes of Scutellaria baicalensis,and to provide reference for the growth of S. baicalensis in large area. METHODS:Seedlings of S. baicalensis potted,8-11 cm in height,were cultured in 1/2 Hoagland nutrient solution with different concentrations of Na Cl. Under simulated salt stress condition,the survival rate of the seedlings was analyzed statistically after 3 days of culture. The contents of chlorophyll and carotenoid were determined by UV visible spectrophotometry,and the content of malonaldehyde was determined by thiobarbituric acid method. The membrane permeability was determined by conductivity method. RESULTS:With the increase of Na Cl concentration,the survival rate of seedling became raised firstly,and declined subsequently,however,the content of MDA and chlorophyll,relative conductivity became raised continuously. The content of carotenoid became raised firstly,and declined subsequently,too. When Na Cl concentration was 0.4%,survival rate and total content of chlorophyll reached its peak. CONCLUSIONS:The critical salt-tolerant concentration for S. baicalensis seedling is 0.4%. When the concentration of Na Cl is less than 0.4%,it has no significant effect on the growth of S. baicalensis seedling;when the concentration of Na Cl is more than 0.4%,serious salt injury have happened.更多还原