【摘要】 目的:建立康普瑞丁A4磷酸酯(CA4P)聚合物囊泡中CA4P的含量测定方法。方法 :采用反相高效液相色谱法。色谱柱为C18,流动相为乙腈-甲醇-0.02 mol/L乙酸铵(10∶40∶50),流速为1.0 ml/min,检测波长为288 nm。结果:CA4P检测质量浓度线性范围为2.1~42μg/ml(r=0.999 9),平均回收率为96.72%(RSD=1.61%,n=3),检测限与定量限分别为0.21、0.70μg/ml。结论:本方法简便、准确度好,可有效控制CA4P聚合物囊泡中CA4P的含量。更多还原

【Abstract】 OBJECTIVE:To establish the method for the content determination of combertastatin A4 phosphate(CA4P)polymersomes vescia. METHODS:RP-HPLC method was adopted. The determination was performed on C18column with mobile phase consisted of acetonitrile-methanol-0.02 mol/L ammonium acetate(10 ∶ 40 ∶ 50)at the flow rate of 1.0 ml/min. The detection wavelength was set at 288 nm. RESULTS:The linear range of CA4P was 2.1-42 μ g/ml(r=0.999 9) with an average recovery of96.72%(RSD=1.61%,n=3). The limits of detection and quantification were 0.21 μg/ml and 0.70 μg/ml. CONCLUSIONS:The method is simple and accurate,and can be used to effectively control the content of CA4P in polymersomes vescia.更多还原