【摘要】 目的:建立消肿接骨贴中苷类成分的定性定量方法。方法:应用薄层色谱(TLC)法对制剂中的续断和黄芪同时进行定性鉴别,以三氯甲烷-甲醇-水(13∶7∶2,V/V/V)的下层溶液为展开剂,10%(V/V)的硫酸乙醇溶液为显色剂。应用高效液相色谱(HPLC)法对制剂中的柚皮苷进行含量测定:色谱柱为Thermo C18(250 mm×4.6 mm,5μm),流动相为乙腈-水(18∶82,V/V),检测波长为283 nm。结果:续断和黄芪的TLC斑点清晰、分离良好,阴性对照无干扰。柚皮苷进样量在0.062 1~0.621 0μg范围内与其峰面积积分值呈良好线性关系(r=0.999 7);精密度、稳定性、重复性试验的RSD<2%;平均加样回收率为99.31%,RSD=1.73%(n=9)。结论:本研究建立的方法专属性强、灵敏度高、简便、准确、重复性好,可以用于消肿接骨贴中苷类成分的定性定量研究。更多还原

【Abstract】 OBJECTIVE:To establish a method for qualitative and quantitative study of glycosides in Xiaozhong jiegu plaster.METHODS:Dipsacus asper and Astragali Radix were identified simultaneously by TLC using chloroform-methyl alcohol-water(13∶ 7∶2,V/V/V)as developing solvent and 10%(V/V)sulfuric acid ethanol as chromogenic agent.HPLC method was used to determine the content of naringin.The determination was performed on Thermo C18(250 mm×4.6 mm,5 μm)with mobile phase consisted of acetonitrile-water(18∶82,V/V)and the detection wavelength was set at 283 nm.RESULTS:TLC spots of D.asper and Astragali Radix were clear and well-separated without interference from negative control.The linear range of naringin was 0.062 1-0.621 0 μg(r=0.999 7)with an average recovery of 99.31%(RSD=1.73%,n=9).RSD of precision,stability and reproducibility tests were all lower than 2%.CONCLUSIONS:The method is specific,sensitive,simple,accurate and reproducible,and it is suitable for qualitative and quantitative identification of glycosides in Xiaozhong jiegu plasters.更多还原