【摘要】 目的探讨苦参碱及苦参碱联合顺铂对人肾母细胞瘤SK-NEP-1细胞存活及凋亡的影响,以及可能的作用机制。方法苦参碱、顺铂单独及联合作用SK-NEP-1细胞,实验分对照组、顺铂干预组、苦参碱干预组、苦参碱联合顺铂干预组。应用四甲基偶氮唑蓝比色法检测SK-NEP-1细胞的存活率,用流式细胞仪检测其凋亡率,逆转录聚合酶链反应法检测其PDCD4 mRNA的表达。结果各组SK-NEP-1细胞的存活率及凋亡率与对照组比较、相同浓度的苦参碱干预组与苦参碱联合顺铂干预组比较及苦参碱联合顺铂组与顺铂干预组比较,SK-NEP-1细胞的存活率及凋亡率差异均有显著性(P<0.05)。各实验组均能提高PDCD4 mRNA的表达,与对照组比较,差异有显著性(P<0.05)。苦参碱联合顺铂组与苦参碱干预组及顺铂干预组比较,SK-NEP-1细胞PDCD4mRNA表达均显著提高(P<0.05)。结论苦参碱可浓度依赖性的抑制SK-NEP-1细胞的增殖并诱导其凋亡,从而提高对顺铂化疗的敏感性,该作用可能与提高细胞内PDCD4 mRNA的表达有关。更多还原

【Abstract】 Objective Abstract:Objective To explore the effects of matrine and cisplatin on survival and apoptosis of nephroblastoma cell line SK-NEP-1,and its potential mechanism.Methods SK-NEP-1 cells were treated with different concentration of Matrine and cisplatin individually or in combination.Trials were divided into following groups:control group,cisplatin intervention group,matrine intervention group,and matrine combined with cisplatin intervention group.The survival rate of SK-NEP-1 cells treated with different concentration of Matrine and cisplatin was detected by MTT colorimetric assay.Apoptosis rate of them by flow cytometry(FCM).RT-PCR analysis was employed to measure the PDCD4 mRNA expression.Results There was significant difference in survival and apoptosis of SK-NEP-1 cells between each group and control group(P < 0.05),The survival rate and apoptosis of SK-NEP-1 cells was statistically significant between Matrine group and Matrine combined with cisplation group(P < 0.05);and also between the Matrine joint cisplation group and cisplation intervention group(P < 0.05).Compared with control group,the expression of PDCD4 mRNA was increased significantly in matrine intervention group,cisplatin intervention group,and matrine combined with cisplatin intervention group(P < 0.05).Combined with matrine and cisplation individual drug group,the expression of PDCD4 mRNA was increased significantly in matrine combined with cisplatin intervention group.Conclusion Matrine could induce apoptosis and inhibit the growth of SK-NEP-1cells in a does-dependent way.Matrine could increase the sensibility of chemotherapy of cisplatin and this effect is correlated to increase expression of PDCD4.更多还原