【摘要】 为调查部分省份牛结核病(bTB)的流行情况,我们在山东等14个省份34个历史上疫情较重的县共129个场户采集牛全血2 201份,采用CO2培养箱或蜡罐方法进行bTBγ-干扰素检测,结果显示样品的个体阳性率为6.4%,群阳性率为42.6%。饲养模式对bTB感染影响的统计显示较大规模场的个体阳性率(6.9%)和群阳性率(60%)均高于散养户(分别为5.7%和35.6%)。而牦牛样品的检测结果均为阴性。5个牛群的禽型PPD阳性统计结果显示牛型PPD阳性率越高,则禽型PPD阳性牛在牛型PPD阳性牛中的所占比率越低。在所有阳性牛群中,阳性率≥10%的牛群所占比例高达81.8%(45/55),bTB感染呈现出一个"群发性"的特征。更多还原

【Abstract】 To investigate the prevalence of bovine tuberculosis(bTB) in some provinces,a total of 2,201 samples of bovine blood were collected from 129 herds with bTB epidemic history in 34 counties of 14 provinces and the plasmas stimulated by bovine PPD,avian PPD and PBS for detection of bovine TB by interferon-γ(INF-γ) test.The results shown that the positives of bovine TB for individual cattle and herds were 6.4% and 42.6%,respectively,detecting the plasma stimulated by bovine PPD in fermenter or CO2incubator.In addition,the positives of bovine TB for individual cattle and herds were 6.9% and 60% in the larger scale farmers which were both higher than in the rural farmers(5.7% and 35.6% respectively).However,no positive sample was detected in yak.While,by detecting the 5 herds,it was shown that the percentage of avian PPD positive to bovine PPD positive increased when bovine PPD positive rate decreased.The character of "group infection" was disclosed as the 81.8% of serious infected herds(≥10%) to all infected herds.更多还原