【摘要】 目的观察去势大鼠阴茎海绵体胱硫醚γ裂解酶(cystathionine-γ-lyase,CSE)/硫化氢(Hydrogen sulphide,H2S)与血红素加氧酶2(heme oxygenase,HO-2)/一氧化碳(carbon monoxide,CO)的交叉调控作用,初步探讨勃起功能障碍的发病机制。方法雄性SD大鼠制备去势大鼠模型,分别施加锌原卟啉(ZnPP,HO阻断剂)和炔丙基甘氨酸(PAG,CSE阻断剂)干预,检测基础条件下和阿扑吗啡(Apomorphine,APO)刺激后的海绵体内压(Intracavernous pressure,ICP)和勃起率;通过Western-blot、敏感硫电极和分光光度计分别检测大鼠勃起不同时期Zn PP(HO阻断剂)对CSE/H2S系统的影响,PAG(CSE阻断剂)对HO-2/CO系统的影响。结果基础条件和APO刺激后去势组、去势PAG组和Zn PP组与假手术组比较,ICP和勃起率下降(P<0.01),且去势PAG组和Zn PP组较去势组ICP明显下降(P<0.01);阿朴吗啡刺激勃起前和勃起中,去势组和去势Zn PP(HO阻断剂)组较假手术组CSE蛋白表达和H2S含量下降(P<0.05),且勃起中去势Zn PP组较去势组CSE蛋白表达和H2S含量明显降低(P<0.05);勃起后去势组和去势ZnPP(HO阻断剂)组较假手术组H2S含量下降(P<0.01)。勃起前和勃起中去势组和去势PAG(CSE阻断剂)组较假手术组HO-2蛋白表达和CO含量下降(P<0.05),且勃起中去势PAG组较去势组HO-2蛋白表达和CO含量明显降低(P<0.05);勃起后去势组和去势PAG(CSE阻断剂)组较假手术组CO含量下降(P<0.01)。勃起前、勃起中和勃起后,CSE和HO-2蛋白表达分别呈正相关(r=0.732,0.850和0.727),H2S和CO水平分别呈正相关(r=0.765,0.912和0.929)。结论 CSE/H2S与HO-2/CO交叉调控作用与去势大鼠勃起功能障碍有关。更多还原

【Abstract】 Objective To explore the cross regulation between CSE / H2 S and HO-2 / CO in the corpus cavernosum of castrated rats in order to study the pathogenesis of erectile dysfunction. Methods Casrated male SD rats were prepared and then randomly injected Zn PP( HO inhibitor) or PAG( CSE inhibitor). Then the ICP and ratio of penile erection under basic condition and after APO induction. The levels of CSE and H2 S in Zn PP blocked group and the levels of HO-2 and CO in PAG blocked group in different period of erection were detected by Western-blot,sulphur-sensitive electrode and spectrophotometer. Results Compared with sham operation group,the ICP and the ratio of penile erection decreased significantly in castration group and castration + PAG / Zn PP group( P < 0. 01);the level of ICP in castration group obviously decreased compared with that in castration + PAG / CSE group. The expression of CSE and the level of H2 S decreased in castration group and in castration + Zn PP group before and during APO induced erection compared with sham operation group; the expression of CSE and the level of H2 S obviously decreased in castration + Zn PP group during erection compared with that in castration group( P < 0. 05). The level of H2 S decreased in castration group and castration + Zn PP group compared with sham operation group after erection( P < 0. 01). The expression of HO-2 and the level of CO decreased in castration group and castration +PAG group compared with that in sham operation group before and during erection( P < 0. 05); compared with castration group,the expression of HO-2 and the level of CO obviously decreased in castration + PAG group during erection( P < 0. 05). Compared with sham operation group,the level of CO decreased in castration group and castration + PAG group after erection( P < 0. 01). In different periods of penile erection( before,during and after erection),the expression of CSE was positively correlated with that of HO-2( r = 0. 732,0. 850 and 0. 727 respectively),and the level of H2 S was positively correlated with that of CO( r = 0. 765,0. 912 and 0. 929 respectively).Conclusion The cross regulation between CSE / H2 S and HO-2 / CO is correlated with erectile dysfunction in castrated rats.更多还原