【摘要】 目的制备血管内皮生长因子(VEGF)可降解缓释微球,考察其生物活性的保存情况以及对血管内皮细胞的作用。方法采用W1/O/W2超声乳化法制备羟基丁酸与羟基辛酸共聚物载血管内皮生长因子纳米微球,采用三步梯度筛网法培养肾微血管内皮细胞,按照培养液中所含成分不同分为3组:血管内皮生长因子组、纳米微球组、对照组,其中前两组血管内皮生长因子的有效质量浓度分别设为10、20、50μg/L。结果培养第1、3天,血管内皮生长因子组与纳米微球组吸光度值比较差异无统计学意义(P>0.05),但吸光度值显著高于对照组(P<0.05),即血管内皮生长因子对肾微血管内皮细胞具有明显促增殖作用;第5、7天纳米微球组吸光度值高于血管内皮生长因子组(P<0.01),即纳米微球缓慢释放血管内皮生长因子,明显提高生物利用度;第7、10天载药纳米微球组微血管内皮细胞仍有较强的增殖能力,与其他两组比较,差异有统计学意义(P<0.01)。结论 VEGF-P(HBHO)NPs对生长因子具有良好缓释作用,比单纯VEGF对肾微血管内皮细胞有更为明显的生物学效应,可以持续促进其增殖。更多还原

【Abstract】 Objective To prepare of vascular endothelial growth factor(VEGF) biodegradable slow-release microspheres,to inspect the preservation of its biological activity and the role of vascular endothelial cells. Methods Hydroxybutyric acid and hydroxy acid copolymer vascular endothelial growth factor nanometer microspheres were prepared by W1/O/W2 ultrasonic emulsification method,the renal microvascular endothelial cells was cultivated by three gradient mesh method,they were divided into three groups according to the nutrient solution contains ingredients different:VEGF group,nanospheres group and control group,among them,the former two groups of vascular endothelial growth factor of the effective mass concentration were set to 10,20,50 μg/L. Results To cultivate 1,3 days,the absorbance value between VEGF group and nanospheres group had no statistical significance(P>0.05),but the absorbance value from them were both higher than that of the control group(P<0.05),VEGF had obvious effect on promoting proliferation of renal microvascular endothelial cells.The absorbance value in nanospheres group was significantly higher than that of the VEGF group at the 5,7 days,nanometer microspheres can slow release of vascular endothelial growth factor and improve bioavailability,microvascular endothelial cells still had a strong ability of proliferation in nanospheres group at the 7,10days,compared with other two groups,the difference was statistically significant(P<0.01). Conclusion VEGF-P(HBHO)NPs has a good slow release effect on growth factors, have a more obvious biological effect than simple VEGF on renal microvascular endothelial cells,it can continue to promote its proliferation.更多还原