【摘要】 目的研究糖尿病慢性肾脏疾病(CKD)患者的HbA1c检测受氨甲酰化血红蛋白(CHb)干扰的情况。方法收集72例CKD患者的标本,分别使用亲和层析法(Primus Ultra2)、离子交换法(VariantⅡ、HA-8160和G7)及参考(IFCC)方法检测。对正常新鲜血进行体外氨甲酰化实验。采用线性回归分析和Bland-Altman方法进行统计学分析。结果血尿素含量逐渐升高,但HbA1c值未相应增加。血红蛋白体外氨甲酰化前后的变化为:HA-8160+0.1%、VariantⅡ+0.4%、G7-0.3%和Primus Ultra2 0%。HA-8160检测值与IFCC方法测定结果最为接近,差值均值接近0,其他方法测定结果均高于IFCC方法。结论 4种方法检测HbA1c均不受CHb的干扰。更多还原

【Abstract】 Objective To investigate the interference of carbamylated hemoglobin(CHb)to HbA1 c test in chronic kidney disease(CKD)of diabetic patients. Methods 72 cases patients with CKD were collected and tested by means of affinity chromatography(Primus Ultra2),ion exchange chromatography(VariantⅡ,HA-8160 and G7)and reference(IFCC)method.In vitro carbamylation experiments were performed.Linear regression and Bland-Altman analysis were also performed. Results With the increase of urea from CKD 3to 5,there was no concurrent increase of HbA1 c.The difference of aftercabarmylation and before-cabarmylation were+0.1%(HA-8160),+0.4%(VariantⅡ),-0.3%(G7)and 0%(Primus Ultra2).Value of HA-8160 was the closest one to IFCC method’s value with difference almost to 0,while the rest ones showed higher values than IFCC method’s value. Conclusion The HbA1 c test is not interfered by the four methods.更多还原