【摘要】 以BORCT蛋白为包被抗原,优化最佳反应条件,确定阈值并测定其交叉反应,建立了一种特异性检测牛巴贝斯虫抗体的间接ELISA方法。利用该方法测定了试验感染的2头牛的抗体动态曲线,并对我国四川、甘肃、新疆3个省(自治区)的314份牛血清样品进行了检测。结果显示,经MedCalc 12.7.3.0软件分析86份阴性血清和69份阳性血清的检测结果,确定29.6%抗体比率为该方法的阳性阈值,对应的敏感性和特异性分别为94.2%和96.5%,并且与其他巴贝斯虫、泰勒虫和无浆体阳性血清均无交叉反应。对试验感染动物的检测结果显示,感染后的第6天出现特异性抗体,第12天达到最高值,一直持续到第270天。野外样品检测结果显示,3个省份均有牛巴贝斯虫的分布,平均阳性率为46.82%。更多还原

【Abstract】 An indirect ELISA for specific detection of Babesia bovis antibodies was established using the purified recombinant protein of B.bovis rhoptry-associated protein 1Cterminal(BORCT)as a coating antigen by optimizing reaction conditions and evaluating specificity and sensitivity.And then ELISA was used to evaluate antibody kinetics of 2experimentally infected cattle by B.bovis and to test 314bovine sera collected from Sichuan,Gansu and Xinjiang Provinces.The results showed that the specificity and sensitivity were 96.5%and 94.2%,respectively,when 29.6%of antibody rate were chosen as a positive threshold via analyzing the data of 86negative sera and 69positive sera with MedCalc 12.7.3.0software.And there were no cross-reactions between BORCT and positive sera of other Babesia,Theileriaand Anaplasmaspecies.Animals experimentally infected with B.bovis had sero-converted by 6days post inoculation and the highest level of antibodies reached after 12days.The specific antibodies lasted for 270days post infection. The test for the field samples demonstrated that B.bovis was distributed in these provinces and the average positive rate of was 46.82%.更多还原