【摘要】 采用皂化/甲酯化GC-MS分析牛脂的脂肪酸组成,主要为棕榈酸(39.86%)、油酸(37.58%)、硬脂酸(11.14%)。采用反相高效液相色谱(RP-HPLC)-大气压化学电离质谱(APCI-MS)及电喷雾电离质谱(ESI-MS)分析牛脂的甘油酯组成,根据ESI准分子离子,APCI准分子离子、二酰基甘油碎片离子、离子阱碰撞诱导解离形成的二级质谱,以及甘油酯在反相色谱上出峰的等价碳原子数原则,并结合脂肪酸组成分析,鉴定出26种甘油酯,包括7种甘二酯和19种甘三酯,其中含量较高的为1-棕榈酸-2-油酸-3-亚油酸甘三酯(11.74%)、1-油酸-2,3-二亚油酸甘三酯(10.97%)、1,3-二油酸-2-亚油酸甘三酯(10.25%)。更多还原

【Abstract】 First the fatty acid composition of tallow was analyzed by GC-MS after saponification and transmethylation. It turned out that palmitic acid(39.86%), oleic acid(37.58%), and stearic acid(11.14%) were the majors. Then reverse phase high performance liquid chromatography(RP-HPLC) coupled with atmospheric pressure chemical ionization mass spectrometry(APCI-MS) as well as electrospray ionization mass spectrometry(ESI-MS) was carried out to analyze glycerides in the tallow. Based on quasi-molecular ion peaks in both APCI and ESI-MS, diacylglycerol fragment ions in APCI-MS, fragment ions in APCI-MS/MS by ion trap collision-induced dissociation, and the equivalence-carbon-number(ECN) rule in elution of glycerides by RP-HPLC as well as the fatty acid composition that had been known, twenty-six glycerides including seven diacylglycerols and nineteen triacylglycerols were identified, among which 1-palmitoyl-2-oleoyl-3-linoleoyl-glycerol(11.74%), 1-oleoyl-2,3-di-linoleoyl-glycerol(10.97%) and 1,3-di-oleoyl 2-linoleoyl-glycerol(10.25%) dominated.更多还原