This paper had obtained complete sequence of DnaK gene from Alicyclobacillus acidoterrestris(DSM3922T) by applying homologous cloning and genome-walking. The DnaK gene contains a complete ORF of 1 854 bp, encoding 617 aa, which deduced amino acid sequence shared the highest homology with DnaK from A. acidocaldarius LAA1(86%). Homology modeling had used to predict the secondary and super-secondary structure. The results showed that A. acidoterrestris DnaK is mainly composed of alpha spiral and beta folding. ...