【摘要】 采用酒石酸铁法测定金丝小枣枣皮、枣核中的多酚类物质含量。首次利用化学发光分析和紫外-可见分光光度分析相结合的方法测定金丝小枣枣皮、枣核中间苯三酚和邻苯二酚的体外抗氧化活性及其清除自由基保护DNA等能力的变化规律。研究结果表明,间苯三酚和邻苯二酚对·OH、NO2-、DPPH具有较明显的抑制作用,对由·OH引起的DNA损伤也具有较好的保护作用。当间苯三酚和邻苯二酚质量浓度为60μg/mL时,体外抗氧化活性及清除自由基保护DNA作用最佳。本研究可为开发枣副产品的保健功能提供新的研究方向和理论基础。更多还原

【Abstract】 The polyphenols content had been measured by tartrate-Fe colorimetry assay from the skin and core of Ziziphus jujuba Mill. cv. Jinsixiaozao. And the antioxidant activity, inhibition ability to hydroxyl free radical, and protective action to DNA of the polyphenols in vitro was determined by using BPCL_L and UV for the first time. The results indicated that phlorglucinol and catechol had inhibition function to hydroxyl free radical, inferior nitric acid rot, DPPH·, besides it had a better protective action to DNA damage caused by ·OH. In addition to that, the 60 μg/mL was the optimal concentration of phlorglucinol and catechol on the antioxidant activity, inhibition function to hydroxyl free radical, and protective action to DNA. Also, it provided scientific basis for re-exploitation and re-application of the products of the Jujuba.更多还原