【摘要】 目的探讨产妇子宫平滑肌大电导钙激活钾通道(BKCa)α、β亚基表达变化在宫缩乏力性产后出血发病中的作用。方法 2009年10月至2011年2月福建省妇幼保健院产科,采用肌条等长张力实验测定30例宫缩乏力性产后出血产妇(病例组)和30例宫缩正常无产后出血产妇(对照组)子宫平滑肌收缩频率、幅度及收缩活动力;应用蛋白印迹法(Western blot)测定两组产妇子宫平滑肌BKCa通道α、β亚基蛋白表达水平;应用实时荧光定量聚合酶链反应(real-time RT-PCR)测定两组产妇子宫平滑肌BKCa通道α、β亚基mRNA表达水平。结果 (1)病例组子宫平滑肌自发宫缩收缩频率、幅度、收缩活动力均低于对照组,分别比较差异均有统计学意义(P<0.05)。(2)病例组子宫平滑肌BKCa通道α、β亚基蛋白表达水平及其mRNA表达水平均高于对照组,分别比较,差异均有统计学意义(P<0.05)。(3)两组产妇子宫平滑肌收缩活动力与BKCa通道α、β亚基蛋白表达水平及BKCa通道α、β亚基mRNA表达水平均呈明显负相关(病例组:r分别为-0.401、-0.727及-0.723、-0.895;对照组:r分别为-0.541、-0.378及-0.703、-0.625;P均<0.05)。结论宫缩乏力性产后出血产妇子宫平滑肌BKCa通道α、β亚基表达水平升高可能是发病的重要因素之一。更多还原

【Abstract】 Objective To detect the expression of large conductance Ca2 +activated K+channel( BKCa) in myometrium in patients with postpartum hemorrhage( PPH). Methods The levels of contractile frequency,amplitude and contraction of myometrium were detected by RM6240 biological experimental system; the BKCa( α,β subunit) protein expression levels in myometrium were detected by Western blot; the BKCa( α,β subunit) mRNA expression levels in myometrium were detected by real-time fluorescent quantitative reverse transcription-polymerase chain reaction for women with PPH caused by uterine atony( study group,n = 30) and normal uterine smooth muscle contraction( control group,n = 30). Results( 1) In study group,the levels of myometrium contractile frequency,amplitude and contraction were lower than that of the controls( P < 0. 05).( 2) In study group,BKCa( α,β subunit) protein and mRNA expression in myometrium were significantly higher than that of control group( P < 0. 05).( 3) Both groups,the level of myometrium contraction was negatively related to BKCa( α,β subunit) protein,BKCa( α,β subunit) mRNA level in myometrium. Conclusion Increased BKCa expression in myometrium may be involved in the pathogenesis of PPH caused by uterine atony.更多还原