【摘要】 目的探讨保胆手术中胆囊壁浆肌层缝合修复胆囊的可行性及其优缺点。方法取实验兔36只雌雄各半,随机分成3组,其中为胆囊壁间断浆肌层缝合组14只、胆囊壁间断全层缝合组14只以及对照组8只。该研究设置对照组只为观察在同等的麻醉、饮食和饲养环境因素条件下胆囊有无结石形成或其他变化,进行统计学数据分析时,不予分析。各组兔按术后7及21 d各取兔一半剖腹观察腹腔情况,并取胆囊缝合处组织,分别行HE染色及Masson染色进行病理学检查。结果①术后所有实验兔均无胆漏;②病理切片表明浆肌层缝合及全层缝合在术后7 d缝合处黏膜已愈合,术后21 d缝合处胶原纤维融合;③间断浆肌层缝合组和间断全层缝合组术后缝合口处组织胆囊壁厚度、炎症反应、成纤维细胞增生情况与胶原纤维含量比较,经统计学分析,差异均无显著性。结论①用兔可以制作胆囊壁浆肌层缝合及全层缝合的模型;②实验证明胆囊壁间断浆肌层缝合与间断全层缝合同样安全、可行。更多还原

【Abstract】 【Objective】To explore the feasibility of seromuscular anastomosis of gallbladder’s wall in rabbit. 【Methods】The thirty-six rabbits were divided into three groups on randomization, seromuscular anastomosis group(n=14), whole-layer anastomosis group(n=14) and control group(n=8). Each rabbit was processed in group in advance to observe the abdominal cavity and the gallbladder was taken postoperative days 7 and days 21, respectively. The sutured tissue was send to pathological examination.【Results】①After postoperative, all experiment rabbits did not have the bile leakage or the abdominal cavity infects; ②The pathological section showed that the mucosa of suture recovered in postoperative days 7, the collagen fibers of suture had been crossed and blending in postoperative days 21; ③Seromuscular anastomosis group and whole-layer anastomosis group after postoperative, the gallbladder wall dropsy situation, inflammation responded(cell proliferation situation, and collagen fiber content were compared without statistical significance. 【Conclusions】①The model of suture the gallbladder wall by seromuscular anastomosis and whole-layer anastomosis can be established with rabbits; ②This experiment proved that is feasibility and safety to suture the gallbladder wall by seromuscular anastomosis and whole-layer anastomosis.更多还原