【摘要】 目的研究糖尿病肾病患者Fractalkine(FKN)受体CX3CR1基因V249I多态性与炎症介质NF-κb、FKN、IL-6、TNF-α的关系。方法应用聚合酶链反应限制片段长度多态性方法及测序法对80例糖尿病肾病(DN组)患者、119例糖尿病(DM组)患者和118例对照者(C组)的CX3CR1基因多态性进行分析,同时检测各组血清中NF-κb、FKN、IL-6、TNF-α含量。结果等位基因249I在对照组(20.33%)中的分布频率明显高于DN组(17.23%)和DM组(8.75%)(χ2=9.698,P=0.002);血清中NF-κb、FKN、IL-6、TNF-α含量DN组高于DM组,DM组高于C组,差异均有统计学意义(各指标F值分别为23.318、52.507、8.821、12.013;P值分别为0.000、0.000、0.001、0.000);DN组及DM组中VI+II基因型FKN含量较VV基因型FKN含量明显增加(F=21.216;P=0.000);DN组及DM组中VI+II基因型NF-κB含量较VV基因型含量明显减少(F=15.361;P=0.000);各组不同基因型中IL-6的含量并无显著差异(F=1.387;P=0.053)。结论 FKN受体CX3CR1等位基因V249I变异可能与糖尿病肾病的发病危险性下降有关;CX3CR1多态性有可能通过影响肾脏的炎症过程而参与DN的发生发展。更多还原

【Abstract】 Objective To investigate the relationship between the polymorphism in fractalkine receptor CX3CR1 gene and inflammation mediators including NF-κB, FKN, IL-6 and TNF-α in diabetic nephropathy(DN) patients. Methods By polymerase chain reaction and restriction fragment length polymorphism(PCRRFLP) and sequencing methods, the V249 I polymorphism in CX3CR1 gene was determined in 80 DN patients, 119 diabetes mellitus(DM) patients and 118 control individuals. The genotype frequency of V249 I polymorphism in CX3CR1 gene was compared among the groups. Serum levels of NF-κB, FKN, IL-6, and TNF-α were measured. Results The allele frequency of V249 I polymorphism was higher in control group(20.33%) than in DN group(8.75%; χ2=9.698, P=0.002) and DM group(17.23%; P< 0.05). Serum levels of NF-κB, FKN, IL-6, TNF-α were higher in DN group than in DM and were higher in DM group than in control group(F=23.318, P<0.001 for NF-κB; F=52.507, P<0.001 for FKN; F=8.821, P=0.001 for IL-6; F=12.013,P<0.001 for TNF-α). In the DN and DM groups, serum FKN increased significantly in patients carrying V/I or I/I genotype than those carrying V/V genotype(F=21.216; P=0.000); serum NF-κB decreased obviously in those carrying V/I or I/I genotype than those carrying V/V genotype(F=15.361; P=0.000); serum IL-6 was unchanged among the patients carrying different genotypes(V/I, I/I and V/V genotypes)(F=1.387; P=0.053).Conclusion The V249 I allele in fractalkine receptor CX3CR1 gene may associate with a lower risk of diabetic nephropathy. This polymorphism in CX3CR1 gene may be involved in the pathogenesis of DN through involving inflammation processes.更多还原