【摘要】 目的研究慢性阻塞性肺疾病(简称慢阻肺)大鼠模型中白细胞介素10(IL-10)、肿瘤坏死因子ɑ(TNF-α)、干扰素-γ(IFN-γ)在血清和肺组织中的表达,探讨IL-10在慢阻肺气道炎症中的作用及调控机制。方法成年雄性SD大鼠45只,分为模型组30只,对照组15只,采用反复熏香烟+气管内注入脂多糖法建立慢阻肺大鼠模型。ELISA法检测大鼠血清、肺组织中IL-10、TNF-ɑ和IFN-γ的表达。结果模型组血清和肺组织内TNF-α较对照组显著升高,IFN-γ、IL-10[血清(44.68±8.67)ng/L比(75.96±10.59)ng/L,肺组织(64.55±9.03)ng/L比(94.06±8.71)ng/L,P均<0.01]表达均下降。IL-10和TNF-ɑ水平呈负相关(血清r=-0.67,肺组织r=-0.80,P<0.01)。IL-10和IFN-γ水平呈正相关(血清r=0.64,肺组织r=0.72,P<0.01)。结论 IL-10的下调在慢阻肺大鼠气道炎症中起重要的调控作用。更多还原

【Abstract】 Objective To investigate the expressions of IL-10,tumor necrosis factor-α(TNF-α) and interferon-γ(IFN-γ) in serum and lung tissue of COPD rats in order to elucidate the potential mechanism of airway inflammation.Methods Forty-five healthy adult male SD rats were randomly divided into a COPD model group(n = 30) and a normal control group(n = 15).The COPD rat model was established by intratracheal instillation of lipopolysaccharide(LPS) and exposure to cigarette smoke for 28 days.The concentrations of IL-10,TNF-α and IFN-γ in serum and lung tissue were measured by ELISA.Results TNF-α level of serum and lung tissue in the COPD model group increased significantly compared with the control group(P < 0.05),while the levels of IFN-γ and IL-10 decreased significantly [serum:(44.68 ± 8.67) ng/L vs.(75.96 ± 10.59) ng/L;lung tissue:(64.55 ± 9.03) ng/L vs.(94.06 ± 8.71) ng / L,P < 0.01].The level of IL-10 in serum and lung tissue was negatively correlated with TNF-α(serum:r =-0.67,lung tissue:r =-0.80,P < 0.01).The level of IL-10 in serum and lung tissue was positively correlated with IFN-γ(serum:r = 0.64,lung tissue:r = 0.72,P < 0.01).The level of IL-10 in serum and lung tissue was negatively correlated with the percentage of neutrophils(serum:r =-0.70,lung tissue:r =-0.67,P < 0.01).Conclusion In COPD rats,down regulation of IL-10 plays an important role in regulation of airway inflammation.更多还原