Objective To explore the distribution of H5 and H9 subtype avian influenza viruses in Shiyan city of Hubei province and to provide evidence for prevention and control of avian influenza. Methods Totally 302 samples were collected from five different typical sites with poultry exposure quarterly in Shiyan in 2012,and then the samples were detected with real-time PCR methods. The data were analyzed with SPSS 17. 0. Results The detection rate of influenza A virus( Flu A) was 26. 82%( 81 /302) for the 5 sites,w...