【摘要】 目的探讨双黄连和清开灵注射剂对血培养的干扰作用。方法将双黄连和清开灵等不同浓度的药液注入血培养瓶中,加入一定浓度的菌液,观察阳性报警时间,并与不加药液的对照组进行比较。结果加入浓度依次为100%,50%清开灵的实验组与对照组相比,ATCC25922阳性报警时间依次延长19%,14%;ATCC25923阳性报警时间依次延长960.6%,136%。加入浓度依次为12.5%,25%,50%,100%双黄连的实验组与对照组相比,ATCC25922阳性报警时间依次延长40%,57%,171%,420%;ATCC25923培养7天均无报警。结论双黄连和清开灵对细菌有不同程度的干扰;双黄连对细菌的干扰作用大于清开灵;采集血培养标本应在应用这两种药物前抽取。更多还原

【Abstract】 Objective To investigate the interference effect of traditional Chinese medicine of Shuanghuanglian and Qingkailing on blood culture.Methods To inject the medicine Shuanghuanglian and Qingkailing of different concentration liquid into blood culture bottle,add a certain concentration of bacterial suspension,observe the positive alarm time and compare with the control group.Results In the experimental group which were treated with Qingkailing of 100%,50% concentration,the positive alarm time of ATCC25922 was averagely increased by 19% and 14% compared with control group,the positive alarm time of ATCC25923 was extended to 960.6% and 136% respectively.In experimental group which were treated with Shuanghuanglian of 12.5%,25%,50%,100% concentration,the positive alarm time of ATCC25922 were extended by 40%,57%,171%,420% compared with the control group,the ATCC25923 was cultured for 7 days with no alarm.Conclusions Shuanghuanglian and Qingkailing injection had a certain interference on bacteria of different degrees,and the intervention function of Shuanghuanglian on bacteria was stronger than that of Qingkailing.Blood cultures should be collected before the application of these two drugs extraction.更多还原